Journal of Surgical Research
Volume 138, Issue 1 , Pages 56-63 , March 2007

Combining siRNAs at Two Different Sites in the EGFR to Suppress its Expression, Induce Apoptosis, and Enhance 5-Fluorouracil Sensitivity of Colon Cancer Cells

  • Xiangbai Wu, M.D.

      Affiliations

    • Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    • ,
  • Yongzhi Deng, M.D.

      Affiliations

    • Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    • ,
  • Guobin Wang, M.D.

      Affiliations

    • Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    • ,
  • Kaixiong Tao, M.D.

      Affiliations

    • Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefangdadao, Hankou, Wuhan, 430022, China.

Received 27 November 2005

References 

  1. Jemal A, Murray T, Samuels A, Ghafoor A, Ward E, Thun MJ. Cancer statistics 2003. CA Cancer J Clin. 2003;53:5
  2. Lu JB, Sun XB, Dai DX, Zhu SK, Chang QL, Liu SZ, et al. Epidemiology of gastroenterologic cancer in Henan Province, China. World J Gastroenterol. 2003;9:2400
  3. Yoshikawa R, Kusunoki M, Yanagi H, Noda M, Furuyama JI, Yamamura T, et al. Dual antitumor effects of 5-fluorouracil on the cell cycle in colorectal carcinoma cells: A novel target mechanism concept for pharmacokinetic modulating chemotherapy. Cancer Res. 2001;61:1029
  4. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998;391:806
  5. Jackson AL, Bartz SR, Schelter J, et al. Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol. 2003;21:635
  6. Aoki Y, Cioca DP, Oidaira H, Kamiya J, Kiyosawa K. RNA interference may be more potent than antisense RNA in human cancer cell lines. Clin Exp Pharmacol Physiol. 2003;30:96
  7. Wells A. EGF receptor. Int J Biochem Cell Biol. 1999;31:637
  8. Arteaga CL. The epidermal growth factor receptor: From mutant oncogene in nonhuman cancers to therapeutic target in human neoplasia. J Clin Oncol. 2001;19:32S
  9. Mendelsohn J, Baselga J. The EGF receptor family as targets for cancer therapy. Oncogene. 2000;19:6550
  10. Nicholson RI, Gee JM, Harper ME. EGFR and cancer prognosis. Eur J Cancer. 2001;37(Suppl):S9
  11. O’Dwyer PJ, Benson AB. Epidermal growth factor receptor-targeted therapy in colorectal cancer. Semin Oncol. 2002;29:10
  12. Fan Z, Masui H, Altas I, Mendelsohn J. Blockade of epidermal growth factor receptor function by bivalent and monovalent fragments of 225 anti-epidermal growth factor receptor monoclonal antibodies. Cancer Res. 1993;53:4322
  13. Gieseg MA, de Bock C, Ferguson LR, Denny WA. Evidence for epidermal growth factor receptor-enhanced chemosensitivity in combinations of cisplatin and the new irreversible tyrosine kinase inhibitor CI-1033. Anticancer Drugs. 2001;12:683
  14. Moulder SL, Yakes FM, Muthuswamy SK, Bianco R, Simpson JF, Arteaga CL. Epidermal growth factor receptor (HER1) tyrosine kinase inhibitor ZD1839 (Iressa) inhibits HER2/neu (erbB2)-overexpressing breast cancer cells in vitro and in vivo. Cancer Res. 2001;61:8887
  15. Norman P. OSI-774 OSI Pharmaceuticals. Curr Opin Investig Drugs. 2001;2:298
  16. Saltz LB, Meropol NJ, Loehrer PJ, Needle MN, Kopit J, Mayer RJ. Phase II trial of cetuximab in patients with refractory colorectal cancer that expresses the epidermal growth factor receptor. J Clin Oncol. 2004;22:1201
  17. Cunningham D, Humblet Y, Siena S, et al. Cetuximab monotherapy and cetuximab plus irinotecan in irinotecan-refractory metastatic colorectal cancer. N Engl J Med. 2004;351:337
  18. Baselga J. The EGFR as a target for anticancer therapy: Focus on cetuximab. Eur J Cancer. 2001;37(Suppl):S16
  19. Bishop PC, Myers T, Robey R, et al. Differential sensitivity of cancer cells to inhibitors of the epidermal growth factor receptor family. Oncogene. 2002;21:119
  20. Yu ZW, Zhao P, Liu M, et al. Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resistant human colon carcinoma LoVo/5-FU cells. World J Gastroenterol. 2004;10:1979
  21. Nyati MK, Maheshwari D, Hanasoge S, et al. Radiosensitization by pan ErbB inhibitor CI-1033 in vitro and in vivo. Clin Cancer Res. 2004;10:691
  22. Tuschl T. Expanding small RNA interference. Nat Biotechnol. 2002;20:446
  23. Zhang M, Zhang X, Bai CX, et al. Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer. Genet Vaccines Ther. 2005;3:5
  24. Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science. 2002;296:550
  25. Bai L, Zhu R, Chen Z, Gao L, Zhang X, Wang X, et al. Potential role of short hairpin RNA targeting epidermal growth factor receptor in growth and sensitivity to drugs of human lung adenocarcinoma cells. Biochem Pharmacol. 2006;71:1265
  26. Pardridge WM. Intravenous, non-viral RNAi gene therapy of brain cancer. Expert Opin Biol Ther. 2004;4:1103
  27. Oldak M, Malejczyk J. [Signal transduction mechanisms induced by epidermal growth factor receptor (EGFR) and their role in apoptosis regulation]. Postepy Hig Med Dosw. 1999;53:315
  28. Harari PM, Huang SM. Head and neck cancer as a clinical model for molecular targeting of therapy: Combining EGFR blockade with radiation. Int J Radiat Oncol Biol Phys. 2001;49:427
  29. Shawver LK, Slamon D, Ullrich A. Smart drugs: Tyrosine kinase inhibitors in cancer therapy. Cancer Cell. 2002;1:117
  30. Huang CY, Ferrell JE. Ultrasensitivity in the mitogen-activated protein kinase cascade. Proc Natl Acad Sci USA. 1996;93:10078

PII: S0022-4804(06)00344-1

doi: 10.1016/j.jss.2006.06.027

Journal of Surgical Research
Volume 138, Issue 1 , Pages 56-63 , March 2007

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