Journal of Surgical Research
Volume 141, Issue 2 , Pages 162-170 , August 2007

RNA Interference Targeting Hypoxia Inducible Factor 1α Reduces Post-Operative Adhesions in Rats

  • Tatiana Segura, Ph.D.

      Affiliations

    • Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Lausanne, Switzerland
    • Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California
    • ,
  • Hugo Schmokel, Ph.D., D.V.M.

      Affiliations

    • Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Lausanne, Switzerland
    • ,
  • Jeffrey A. Hubbell, Ph.D.

      Affiliations

    • Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Lausanne, Switzerland
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at: Jeffrey A. Hubbell, EPFL SV-LMRP, Station 15, CH-1015 Lausanne, Switzerland.

Received 3 April 2006

References 

  1. Menzies D, Ellis H. Intestinal obstruction from adhesions: How big is the problem?. Ann R Coll Surg Engl. 1990;72:60
  2. Menzies D. Postoperative adhesions: Their treatment and relevance in clinical practice. Ann R Coll Surg Engl. 1993;75:147
  3. Diamond MP, Hershlag A. Adhesion formation/reformation. Prog Clin Biol Res. 1990;358:23
  4. Holmdahl L, Eriksson E, al-Jabreen M, et al. Fibrinolysis in human peritoneum during operation. Surgery. 1996;119:701
  5. Boland GM, Weigel RJ. Formation and prevention of postoperative abdominal adhesions. J Surg Res. 2006;132:3;Epub 2006 Feb 2.
  6. Risberg B. Adhesions: Preventive strategies. Eur J Surg. 1997;(Suppl):32
  7. Montz FJ, Fowler JM, Wolff AJ, et al. The ability of recombinant tissue plasminogen activator to inhibit post-radical pelvic surgery adhesions in the dog model. Am J Obstet Gynecol. 1991;165:1539
  8. Dunn RC, Mohler M. Effect of varying days of tissue plasminogen activator therapy on the prevention of postsurgical adhesions in a rabbit model. J Surg Res. 1993;54:242
  9. Hill-West JL, Dunn RC, Hubbell JA. Local release of fibrinolytic agents for adhesion prevention. J Surg Res. 1995;59:759
  10. Falk K, Bjorquist P, Stromqvist M, et al. Reduction of experimental adhesion formation by inhibition of plasminogen activator inhibitor type 1. Br J Surg. 2001;88:286
  11. Fukui N, Tashiro T, Hiraoka H, et al. Adhesion formation can be reduced by the suppression of transforming growth factor-beta1 activity. J Orthop Res. 2000;18:212
  12. Lucas PA, Warejcka DJ, Young HE, et al. Formation of abdominal adhesions is inhibited by antibodies to transforming growth factor-beta1. J Surg Res. 1996;65:135
  13. Saltzman AK, Olson TA, Mohanraj D, et al. Prevention of postoperative adhesions by an antibody to vascular permeability factor/vascular endothelial growth factor in a murine model. Am J Obstet Gynecol. 1996;174:1502
  14. Ivarsson ML, Bergstrom M, Eriksson E, et al. Tissue markers as predictors of postoperative adhesions. Br J Surg. 1998;85:1549
  15. Holmdahl L, Kotseos K, Bergstrom M, et al. Overproduction of transforming growth factor-beta1 (TGF-beta1) is associated with adhesion formation and peritoneal fibrinolytic impairment. Surgery. 2001;129:626
  16. Fitzpatrick TE, Graham CH. Stimulation of plasminogen activator inhibitor-1 expression in immortalized human trophoblast cells cultured under low levels of oxygen. Exp Cell Res. 1998;245:155
  17. Uchiyama T, Kurabayashi M, Ohyama Y, et al. Hypoxia induces transcription of the plasminogen activator inhibitor-1 gene through genistein-sensitive tyrosine kinase pathways in vascular endothelial cells. Arterioscler Thromb Vasc Biol. 2000;20:1155
  18. Kietzmann T, Roth U, Jungermann K. Induction of the plasminogen activator inhibitor-1 gene expression by mild hypoxia via a hypoxia response element binding the hypoxia-inducible factor-1 in rat hepatocytes. Blood. 1999;94:4177
  19. Zhang Q, Wu Y, Chau CH, et al. Crosstalk of hypoxia-mediated signaling pathways in upregulating plasminogen activator inhibitor-1 expression in keloid fibroblasts. J Cell Physiol. 2004;199:89
  20. Kietzmann T, Samoylenko A, Roth U, et al. Hypoxia-inducible factor-1 and hypoxia response elements mediate the induction of plasminogen activator inhibitor-1 gene expression by insulin in primary rat hepatocytes. Blood. 2003;101:907
  21. Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001;411:494
  22. Schiffelers RM, Ansari A, Xu J, et al. Cancer siRNA therapy by tumor selective delivery with ligand-targeted sterically stabilized nanoparticle. Nucleic Acids Res. 2004;32:e149
  23. Urban-Klein B, Werth S, Abuharbeid S, et al. RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo. Gene Ther. 2005;12:461
  24. Banerjea A, Li MJ, Bauer G, et al. Inhibition of HIV-1 by lentiviral vector-transduced siRNAs in T lymphocytes differentiated in SCID-hu mice and CD34+ progenitor cell-derived macrophages. Mol Ther. 2003;8:62
  25. Wu CJ, Huang HW, Liu CY, et al. Inhibition of SARS-CoV replication by siRNA. Antiviral Res. 2005;65:45
  26. Soutschek J, Akinc A, Bramlage B, et al. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature. 2004;432:173
  27. Zender L, Hutker S, Liedtke C, et al. Caspase 8 small interfering RNA prevents acute liver failure in mice. Proc Natl Acad Sci USA. 2003;100:7797
  28. Hassani Z, Lemkine GF, Erbacher P, et al. Lipid-mediated siRNA delivery down-regulates exogenous gene expression in the mouse brain at picomolar levels. J Gene Med. 2005;7:198
  29. Xia H, Mao Q, Eliason SL, et al. RNAi suppresses polyglutamine-induced neurodegeneration in a model of spinocerebellar ataxia. Nat Med. 2004;10:816
  30. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25:402
  31. Chowdhury SM, Hubbell JA. Adhesion prevention with ancrod released via a tissue-adherent hydrogel. J Surg Res. 1996;61:58
  32. Winblade ND, Schmokel H, Baumann M, et al. Sterically blocking adhesion of cells to biological surfaces with a surface-active copolymer containing poly(ethylene glycol) and phenylboronic acid. J Biomed Mater Res. 2002;59:618
  33. Elbert DL, Hubbell JA. Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces. J Biomed Mater Res. 1998;42:55
  34. Chegini N. The role of growth factors in peritoneal healing: Transforming growth factor beta (TGF-beta). Eur J Surg Suppl. 1997;17:
  35. Chegini N, Kotseos K, Bennett B, et al. Matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in peritoneal fluids and sera and correlation with peritoneal adhesions. Fertil Steril. 2001;76:1207
  36. Chegini N, Kotseos K, Zhao Y, et al. Expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in serosal tissue of intraperitoneal organs and adhesions. Fertil Steril. 2001;76:1212
  37. Molinas CR, Mynbaev O, Pauwels A, et al. Peritoneal mesothelial hypoxia during pneumoperitoneum is a cofactor in adhesion formation in a laparoscopic mouse model. Fertil Steril. 2001;76:560
  38. Molinas CR, Campo R, Elkelani OA, et al. Role of hypoxia inducible factors 1alpha and 2alpha in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic mice. Fertil Steril. 2003;80(Suppl):795
  39. Tietze L, Elbrecht A, Schauerte C, et al. Modulation of pro- and antifibrinolytic properties of human peritoneal mesothelial cells by transforming growth factor beta1 (TGF-beta1), tumor necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta). Thromb Haemost. 1998;79:362
  40. Krishnamachary B, Berg-Dixon S, Kelly B, et al. Regulation of colon carcinoma cell invasion by hypoxia-inducible factor 1. Cancer Res. 2003;63:1138
  41. Graham CH, Fitzpatrick TE, McCrae KR. Hypoxia stimulates urokinase receptor expression through a heme protein-dependent pathway. Blood. 1998;91:3300
  42. Kimura D, Imaizumi T, Tamo W, et al. Hypoxia enhances the expression of plasminogen activator inhibitor-1 in human lung cancer cells, EBC-1. Tohoku J Exp Med. 2002;196:259
  43. Saed GM, Zhang W, Chegini N, et al. Transforming growth factor beta isoforms production by human peritoneal mesothelial cells after exposure to hypoxia. Am J Reprod Immunol. 2000;43:285
  44. Border WA, Ruoslahti E. Transforming growth factor-beta in disease: The dark side of tissue repair. J Clin Invest. 1992;90:1
  45. Massague J. The transforming growth factor-beta family. Annu Rev Cell Biol. 1990;6:597
  46. Lungwitz U, Breunig M, Blunk T, et al. Polyethylenimine-based non-viral gene delivery systems. Eur J Pharm Biopharm. 2005;60:247

PII: S0022-4804(06)00418-5

doi: 10.1016/j.jss.2006.07.045

Journal of Surgical Research
Volume 141, Issue 2 , Pages 162-170 , August 2007

Article Tools

* Image Usage & Resolution