Journal of Surgical Research
Volume 144, Issue 1 , Pages 59-63 , January 2008

The Effect of Ethyl Pyruvate on Oxidative Stress in Intestine and Bacterial Translocation After Thermal Injury

  • Melih Karabeyoğlu, M.D.

      Affiliations

    • Department of 2nd General Surgery, Numune Education and Research Hospital, Ankara, Turkey
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at Bascavus Sok. Ugur Apt. 20/4, Kucukesat, Cankaya, 06660, Ankara, Turkey.
  • ,
  • Bülent Ünal, M.D.

      Affiliations

    • Department of 2nd General Surgery, Numune Education and Research Hospital, Ankara, Turkey
  • ,
  • Betül Bozkurt, M.D.

      Affiliations

    • Department of 2nd General Surgery, Numune Education and Research Hospital, Ankara, Turkey
  • ,
  • Iştar Dolapçı, M.D.

      Affiliations

    • Department of Microbiology, Faculty of Medicine, Ankara University, Ankara, Turkey
  • ,
  • Ayşe Bilgihan

      Affiliations

    • Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey
  • ,
  • Işıl Karabeyoğlu, M.D.

      Affiliations

    • Department of Anesthesiology and Reanimation, Numune Education and Research Hospital, Ankara, Turkey
  • ,
  • Ümer Cengiz, M.D.

      Affiliations

    • Department of 2nd General Surgery, Numune Education and Research Hospital, Ankara, Turkey

Received 2 November 2006

References 

  1. Sitting K, Deitch EA. Effect of bacteremia on mortality after thermal injury. Arch Surg. 1988;123:1367
  2. Deitch EA, Berg R. Bacterial translocation from the gut: A mechanism of infection. J Burn Care Rehabil. 1987;8:475
  3. Tokyay R, Zeigler ST, Traber DL, et al. Postburn gastrointestinal vasoconstriction increases bacterial and endotoxin translocation. J Appl Physiol. 1993;74:1521
  4. Willy C, Dahouk S, Starek C, et al. DNA damage in humane leucocytes after ischemia/reperfusion injury. Free Radic Biol Med. 2000;28:1
  5. Mathru M, Rooney MW, Dries DJ, et al. Urine hydrogen peroxide during adult respiratory distress syndrome in patient with and with-out sepsis. Chest. 1994;105:232
  6. Sprong RC, Aarsman CJ, Van Oirschot JF, et al. Dimethylthiourea protects rats against Gram-negative sepsis and decreases tumor necrosis factor and nuclear factor kappa B activity. J Lab Clin Med. 1997;129:470
  7. Jacobs DM, Julsud JM, Bubrick MP. Iron chelation with deferoxamine conjugate in hemorrhagic shock. J Surg Res. 1991;51:484
  8. Lalonde C, Nayak U, Hennigan J, et al. Anti-oxidants prevent the cellular deficit produced in response to burn injury. J Burn Care Rehabil. 1996;17:379
  9. Carrie AS, Somkiat W, Michael JM, et al. Ringer’s ethyl pyruvate solution ameliorates ischemia-reperfusion-induced intestinal mucosal injury in rats. Crit Care Med. 2001;29:1513
  10. Fink MP. Ethyl pyruvate: A novel anti-inflammatory agent. Crit Care Med. 2003;31:51
  11. Zingarelli B. Editorial: Ethyl pyruvate: A simple solution?. Crit Care Med. 2004;32:1603
  12. Dobsak P, Courderdot-Masuyer C, Zeller M, et al. Antioxidative properties of pyruvate and protection of the ischemic rat heart during cardioplegia. J Cardiovasc Pharmacol. 1999;34:651
  13. Bunger R, Mallet RT, Hartman DA. Pyruvate-enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart: Near complete prevention of reperfusion contractile failure. Eur J Biochem. 1989;180:221
  14. Cicalese L, Lee K, Schraut W, et al. Pyruvate prevents ischemia-reperfusion mucosal injury in rat small intestine. Am J Surg. 1999;171:97
  15. Sileri P, Schena S, Morini S, et al. Pyruvate inhibits hepatic ischemia-reperfusion injury in rats. Transplantation. 2001;72:27
  16. Sims CA, Wattanasirichaigoon S, Menconi MJ, et al. Ringer’s ethyl pyruvate solution ameliorates ischemia/reperfusion-induced intestinal mucosal injury in rats. Crit Care Med. 2001;29:1513
  17. Walker HL, Maso AD. A standard animal burn. J Trauma. 1968;8:1049
  18. Grisham MB, Hernandez LA, Granger DN. Xhanthine oxidase neutrophil infiltration in intestinal ischemia. Am J Physiol. 1987;251:49
  19. Glowick SP, Kaplan SD. Methods in Enzymology. In: New York: Academic Press; 1955;p. 769
  20. Uchiyama M, Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Ann Biochem. 1978;86:271
  21. Carden DL, Granger DN. Pathophysiology of ischemia-reperfusion injury. J Pathol. 2000;190:255
  22. Sener G, Sehirli AO, Satiroglu H, et al. Melatonin improves oxidative organ damage in a rat model of thermal injury. Burns. 2002;28:419
  23. Horton JW. Free radicals and lipid peroxidation mediated injury in burn trauma: The role of antioxidant therapy. Toxicology. 2003;189:75
  24. Sehitli AO, Sener G, Satiroglu H, et al. Protective effect of N-acetylcysteine on renal ischemia-reperfusion injury in rat. J Nephrol. 2003;16:75
  25. Youn Y-K, Suh GJ, Jung SE, et al. Recombinant human growth hormone decreates lung and liver lipid peroxidation and increases antioxidant activity after thermal injury in rats. J Burn Care Rehabil. 1998;19:542
  26. Uchiyama T, Delude RL, Fink MP. Dose-dependent effects of ethyl pyruvate in mice subjected to mesenteric ischemia and reperfusion. Intensive Care Med. 2003;29:2050
  27. Yang R, Gallo DJ, Baust JJ, et al. Ethyl pyruvate modulates inflammatory gene expression in mice subjected to hemorrhagic shock. Am J Physiol Gastrointest Liver Physiol. 2002;283:212
  28. Ulloa L, Ochani M, Yang H, et al. Ethyl pyruvate prevents lethality in mice with established lethal sepsis and systemic inflammation. Proc Natl Acad Sci USA. 2002;99:12351
  29. Miyaji T, Hu X, Yuen PST, et al. Ethyl pyruvate decreases sepsis-induced acute renal failure and multiple organ damage in aged mice. Kidney Int. 2003;64:1620
  30. Yang R, Uchiyama T, Alber SM, et al. Ethyl pyruvate ameliorates distant organ injury in a murine model of acute necrotizing pancreatitis. Crit Care Med. 2004;32:1453
  31. Yang R, Han X, Delude RL, et al. Ethyl pyruvate ameliorates acute alcohol-induced liver injury and inflammation in mice. J Lab Clin Med. 2003;142:322, 212
  32. Wang WJ, Yao YM, Xian LM, et al. Effect of treatment with ethyl pyruvate on multiple organ dysfunction and mortality following delayed resuscitation after burn injury in rat. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2006;18:132
  33. Dong N, Yao YM, Dong YQ, et al. Effects of ethyl pyruvate on splenocyte proliferation and apoptosis in burn rats with delayed resuscitation. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005;17:393
  34. Dong YQ, Yao YM, Wei P, et al. Effects of ethyl pyruvate on cell-mediated immune function in rats with delayed resuscitation after burn injury. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005;17:12
  35. Cuzzocrea S, Zingarelli B, Costantino G, et al. Beneficial effects of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase in a rat model of splanchnic artery occlusion and reperfusion. Br J Pharmacol. 1997;121:1065
  36. Harada T, Moore BA, Yang R, et al. Ethyl pyruvate ameliorates ileus induced by bowel manipulation in mice. Surgery. 2005;138:530
  37. Song M, Kellum JA, Kaldas H, et al. Evidence that glutathione depletion is a mechanism responsible for the anti-inflammatory effects of ethyl pyruvate in cultured LPS-stimulated RAW 264.7 cells. J Pharmacol Exp Ther. 2004;308:307
  38. Kalff JC, Turler A, Schwarz NT, et al. Intra-abdominal activation of a local inflammatory response within the human muscularis externa during laparotomy. Ann Surg. 2003;237:316
  39. Andersson U, Wang H, Palmblad K, et al. High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes. J Exp Med. 2000;21:192:565
  40. Droge W, Mihm S, Bockstette M, et al. Effect of reactive oxygen intermediates and antioxidants on proliferation and function of T lymphocytes. Methods Enzymol. 1994;34:135
  41. Kefer J, Rahman A, Anwar KN, et al. Decreased oxidant buffering impairs NF-kappaB activation and ICAM-1 transcription in endothelial cells. Shock. 2001;15:11

PII: S0022-4804(07)00167-9

doi: 10.1016/j.jss.2007.02.050

Journal of Surgical Research
Volume 144, Issue 1 , Pages 59-63 , January 2008