Review of Various Techniques of Pancreas Transplantation in Rat Model

Mood, Zhoobin A.; Mehrabi, Arianeb; Schmied, Bruno M.; Müller, Sascha A.; Engelmann, Guido; Schemmer, Peter; Weitz, Jürgen; Büchler, Markus W.; Gebhard, Martha M.; Schmidt, Jan

doi:10.1016/j.jss.2007.03.027

« Previous Next »Journal of Surgical Research
Volume 145, Issue 2 , Pages 205-213, April 2008

Review of Various Techniques of Pancreas Transplantation in Rat Model1

Zhoobin A. Mood, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Arianeb Mehrabi, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
- To whom correspondence and reprint requests should be addressed at Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg. Germany.
Bruno M. Schmied, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Sascha A. Müller, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Guido Engelmann, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Peter Schemmer, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Jürgen Weitz, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Markus W. Büchler, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Martha M. Gebhard, M.D.
- Department of Experimental Surgery, University of Heidelberg, Heidelberg, Germany
Jan Schmidt, M.D.
- Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany

Received 12 December 2006 published online 03 September 2007.

Pancreas transplantation (PTx) is being applied with increasing frequency in the treatment of diabetes mellitus Type 1 and selected cases of Type 2. It is known that PTx can consistently establish normoglycemic insulin-independent state. Due to the increased use of different PTx models in rats for studying the metabolic function of the transplanted pancreas, reviewing the various techniques in PTx seemed to be necessary.

A review of the literature of PTx in rat models showed that different techniques or modifications have been described. Each modification is the result of a change or simplification of three main points: the arterial inflow, the venous outflow, and the management of the exocrine secretory part of the gland.

The techniques of PTx vascularization in rats are based on two cardinal models, which include the microsuture and non-suture cuff techniques. In arterial inflow reconstruction, an aortic segment is used. Venous drainage is accomplished by systemic or portal drainage (porto-caval or porto-portal anastomosis). Management of the exocrine part has the most variations. Enteric and urinary diversion, as well as duct ligation or obstruction, are the most important techniques for the management of exocrine part.

Regarding vascular anastomosis, the microsuture technique is more popular than the cuff technique. However the cuff technique seems to be simpler. Grafts with portal venous drainage show a lesser probability of hyperinsulinemia and a high level of lipoproteins. In the management of the exocrine part, enteric exocrine drainage is more similar to the physiological setting and is associated with a significant reduction in the incidence of acidosis and dehydration. However, enteric diversion is more prone to intestinal bleeding and ileus as well as to bacterial contamination. The technique for PTx in rat varies according to surgeons’ experiences and preferences as well as their research objectives.

Key Words: pancreas transplantation, rat model, various techniques, complications, contributing factors, advantages and disadvantages, review