Journal of Surgical Research
Volume 155, Issue 2 , Pages 183-190 , August 2009

Detection of Epithelial Cells by RT-PCR Targeting CEA, CK20, and TEM-8 in Colorectal Carcinoma Patients Using OncoQuick Density Gradient Centrifugation System

  • Emmanuel E. Lagoudianakis, M.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
  • ,
  • Agapi Kataki, Ph.D.

      Affiliations

    • Laboratory of Surgical Research, First Department of Propaedeutic Surgery, Hippocrateion Hospital of Athens, Athens Medical School, Athens, Greece
  • ,
  • Andreas Manouras, M.D., Ph.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Q. Sophia 14, 11527, Athens, Greece
  • ,
  • Nikolaos Memos, M.D.

      Affiliations

    • Laboratory of Surgical Research, First Department of Propaedeutic Surgery, Hippocrateion Hospital of Athens, Athens Medical School, Athens, Greece
  • ,
  • Artemisia Papadima, M.D.

      Affiliations

    • Department of Anesthesiology, Hippocrateion Hospital, Athens, Greece
  • ,
  • Anastasia Derventzi, Ph.D.

      Affiliations

    • Laboratory of Surgical Research, First Department of Propaedeutic Surgery, Hippocrateion Hospital of Athens, Athens Medical School, Athens, Greece
  • ,
  • George Zografos, M.D., Ph.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
  • ,
  • Stefanos Papadopoulos, M.D., Ph.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
  • ,
  • Vaggelogiannis Katergiannakis, M.D., Ph.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece
  • ,
  • Manoussos M. Konstadoulakis, M.D., Ph.D.

      Affiliations

    • First Department of Propaedeutic Surgery, Hippocrateion Hospital, Athens Medical School, Athens, Greece

Received 7 May 2007

References 

  1. Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2006. CA Cancer J Clin. 2006;56:106
  2. Wingo PA, Ries LA, Rosenberg HM, et al. Cancer incidence and mortality, 1973–1995: A report card for the U.S.. Cancer. 1998;82:1197
  3. Read TE, Mutch MG, Chang BW, et al. Locoregional recurrence and survival after curative resection of adenocarcinoma of the colon. J Am Coll Surg. 2002;195:33
  4. Koch M, Kienle P, Hinz U, et al. Detection of hematogenous tumor cell dissemination predicts tumor relapse in patients undergoing surgical resection of colorectal liver metastases. Ann Surg. 2005;241:199
  5. Hardingham JE, Hewett PJ, Sage RE, et al. Molecular detection of blood-borne epithelial cells in colorectal cancer patients and in patients with benign bowel disease. Int J Cancer. 2000;89:8
  6. Rosenberg R, Hoos A, Mueller J, et al. Impact of cytokeratin-20 and carcinoembryonic antigen mRNA detection by RT-PCR in regional lymph nodes of patients with colorectal cancer. Br J Cancer. 2000;83:1323
  7. Schuster R, Max N, Mann B, et al. Quantitative real-time RT-PCR for detection of disseminated tumor cells in peripheral blood of patients with colorectal cancer using different mRNA markers. Int J Cancer. 2004;108:219
  8. Giribaldi G, Procida S, Ulliers D, et al. Specific detection of cytokeratin 20-positive cells in blood of colorectal and breast cancer patients by a high sensitivity real-time reverse transcriptase-polymerase chain reaction method. J Mol Diagn. 2006;8:105
  9. Funaki NO, Tanaka J, Itami A, et al. Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA. Life Sci. 1997;60:643
  10. Ikeguchi M, Ohro S, Maeda Y, et al. Detection of cancer cells in the peripheral blood of gastric cancer patients. Int J Mol Med. 2003;11:217
  11. Noura S, Yamamoto H, Ohnishi T, et al. Comparative detection of lymph node micrometastases of stage II colorectal cancer by reverse transcriptase polymerase chain reaction and immunohistochemistry. J Clin Oncol. 2002;20:4232
  12. Guadagni F, Kantor J, Aloe S, et al. Detection of blood-borne cells in colorectal cancer patients by nested reverse transcription-polymerase chain reaction for carcinoembryonic antigen messenger RNA: Longitudinal analyses and demonstration of its potential importance as an adjunct to multiple serum markers. Cancer Res. 2001;61:2523
  13. Ichikawa D, Kitamura K, Tani N, et al. Molecular detection of disseminated cancer cells in the peripheral blood and expression of sialylated antigens in colon cancers. J Surg Oncol. 2000;75:98
  14. Nanda A, St Croix B. Tumor endothelial markers: New targets for cancer therapy. Curr Opin Oncol. 2004;16:44
  15. Rmali KA, Watkins G, Harrison G, et al. Tumor endothelial marker 8 (TEM-8) in human colon cancer and its association with tumor progression. Eur J Surg Oncol. 2004;30:948
  16. Rmali KA, Puntis MC, Jiang WG. Prognostic values of tumor endothelial markers in patients with colorectal cancer. World J Gastroenterol. 2005;11:1283
  17. Mori M, Mimori K, Ueo H, et al. Clinical significance of molecular detection of carcinoma cells in lymph nodes and peripheral blood by reverse transcription-polymerase chain reaction in patients with gastrointestinal or breast carcinomas. J Clin Oncol. 1998;16:128
  18. Xu D, Li XF, Zheng S, Jiang WZ. Quantitative real-time RT-PCR detection for CEA (CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients). J Zhejiang Univ Sci B. 2006;7:445
  19. Huang P, Wang J, Guo Y, et al. Molecular detection of disseminated tumor cells in the peripheral blood in patients with gastrointestinal cancer. J Cancer Res Clin Oncol. 2003;129:192
  20. Chausovsky G, Luchansky M, Figer A, et al. Expression of cytokeratin 20 in the blood of patients with disseminated carcinoma of the pancreas, colon, stomach, and lung. Cancer. 1999;86:2398
  21. Silva JM, Rodriguez R, Garcia JM, et al. Detection of epithelial tumor RNA in the plasma of colon cancer patients is associated with advanced stages and circulating tumor cells. Gut. 2002;50:530
  22. Fidler IJ. Metastasis: Quantitative analysis of distribution and fate of tumor embolilabeled with 125 I-5-iodo-2′-deoxyuridine. J Natl Cancer Inst. 1970;45:773
  23. Mizuno N, Kato Y, Shirota K, et al. Mechanism of initial distribution of blood-borne colon carcinoma cells in the liver. J Hepatol. 1998;28:878
  24. Patel H, Le Marer N, Wharton RQ, et al. Clearance of circulating tumor cells after excision of primary colorectal cancer. Ann Surg. 2002;235:226
  25. Anker P, Mulcahy H, Chen XQ, et al. Detection of circulating tumour DNA in the blood (plasma/serum) of cancer patients. Cancer Metastasis Rev. 1999;18:65
  26. Tien YW, Lee PH, Wang SM, et al. Simultaneous detection of colonic epithelial cells in portal venous and peripheral blood during colorectal cancer surgery. Dis Colon Rectum. 2002;45:23
  27. Nagao K, Hisatomi H, Hirata H, et al. Expression of molecular marker genes in various types of normal tissue: Implication for detection of micrometastases. Int J Mol Med. 2002;10:307
  28. Jonas S, Windeatt S, O-Boateng A, et al. Identification of carcinoembryonic antigen-producing cells circulating in the blood of patients with colorectal carcinoma by reverse transcriptase polymerase chain reaction. Gut. 1996;39:717
  29. Sadahiro S, Suzuki T, Maeda Y, et al. Detection of Carcinoembryonic antigen messenger RNA-expressing cells in peripheral blood 7 days after curative surgery is a novel prognostic factor in colorectal cancer. Ann Surg Oncol. 2007;14:1092
  30. Wang JY, Wu CH, Lu CY, et al. Molecular detection of circulating tumor cells in the peripheral blood of patients with colorectal cancer using RT-PCR: Significance of the prediction of postoperative metastasis. World J Surg. 2006;30:1007
  31. Iinuma H, Okinaga K, Egami H, et al. Usefulness and clinical significance of quantitative real-time RT-PCR to detect isolated tumor cells in the peripheral blood and tumor drainage blood of patients with colorectal cancer. Int J Oncol. 2006;28:297
  32. Sadahiro S, Suzuki T, Ishikawa K, et al. Detection of carcinoembryonic antigen messenger RNA-expressing cells in portal and peripheral blood during surgery does not influence relapse in colorectal cancer. Ann Surg Oncol. 2005;12:988
  33. Stathopoulou A, Mavroudis D, Perraki M, et al. Molecular detection of cancer cells in the peripheral blood of patients with breast cancer: Comparison of CK-19, CEA and maspin as detection markers. Anticancer Res. 2003;23:1883
  34. Oberg AN, Lindmark GE, Israelsson AC, et al. Detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT-PCR for CEA and CK20 mRNAs. Int J Cancer. 2004;111:101
  35. Champelovier P, Mongelard F, Seigneurin D. CK20 gene expression: Technical limits for the detection of circulating tumor cells. Anticancer Res. 1999;19:2073
  36. Liu S, Leppla SH. Cell surface tumor endothelium marker 8 cytoplasmic tail-independent anthrax toxin binding, proteolytic processing, oligomer formation, and internalization. J Biol Chem. 2003;278:5227
  37. Colombatti A, Bonaldo P. The superfamily of proteins with von Willebrand factor type A-like domains: One theme common to components of extracellular matrix, hemostasis, cellular adhesion, and defense mechanisms. Blood. 1991;77:2305
  38. Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res. 2001;61:6649
  39. Rmali KA, Al-Rawi MA, Parr C, et al. Up-regulation of tumor endothelial marker-8 by interleukin-1beta and its impact in IL-1beta induced angiogenesis. Int J Mol Med. 2004;14:75
  40. Gertler R, Rosenberg R, Fuehrer K, et al. Detection of circulating tumor cells in blood using an optimized density gradient centrifugation. Recent Results Cancer Res. 2003;162:149
  41. Lacroix J, Doeberitz MK. Technical aspects of minimal residual disease detection in carcinoma patients. Semin Surg Oncol. 2001;20:252
  42. Henke W, Loening SA. Detection of illegitimate transcripts of prostate-specific antigen mRNA in blood by reverse transcription-polymerase chain reaction. Int J Cancer. 1998;77:164
  43. Rosenberg R, Gertler R, Friederichs J, et al. Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood. Cytometry. 2002;49:150
  44. Ito S, Nakanishi H, Hirai T, et al. Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with real-time RT-PCR on a LightCycler. Cancer Lett. 2002;183:195
  45. Sadahiro S, Suzuki T, Tokunaga N, et al. Detection of tumor cells in the portal and peripheral blood of patients with colorectal carcinoma using competitive reverse transcriptase-polymerase chain reaction. Cancer. 2001;92:1251
  46. Gimbrone MA, Leapman SB, Cotran RS, et al. Tumor dormancy in vivo by prevention of neovascularization. J Exp Med. 1972;136:261
  47. Ferrara N. VEGF and the quest for tumor angiogenesis factors. Nat Rev Cancer. 2002;2:795
  48. Blood CH, Zetter BR. Tumor interactions with the vasculature: Angiogenesis and tumor metastasis. Biochim Biophys Acta. 1990;1032:89
  49. Butler TP, Gullino PM. Quantitation of cell shedding into efferent blood of mammary adenocarcinoma. Cancer Res. 1975;35:512
  50. Chambers AF, Groom AC, MacDonald IC. Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer. 2002;2:563
  51. Fidler IJ. The pathogenesis of cancer metastasis: The “seed and soil” hypothesis revisited. Nat Rev Cancer. 2003;3:453
  52. Luzzi KJ, MacDonald IC, Schmidt EE, et al. Multistep nature of metastatic inefficiency: Dormancy of solitary cells after successful extravasation and limited survival of early micrometastases. Am J Pathol. 1998;3:865;15
  53. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: A new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst. 1992;84:1875
  54. Fox SH, Whalen GF, Sanders MM, et al. Angiogenesis in normal tissue adjacent to colon cancer. J Surg Oncol. 1998;69:230

PII: S0022-4804(07)00635-X

doi: 10.1016/j.jss.2007.10.013

Journal of Surgical Research
Volume 155, Issue 2 , Pages 183-190 , August 2009