Journal of Surgical Research
Volume 149, Issue 2 , Pages 296-302 , October 2008

Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Inducible Nitric Oxide Synthase and Nitric Oxide Production Is Decreased by an Omega-3 Fatty Acid Lipid Emulsion

  • Christopher Aldridge, B.A.

      Affiliations

    • Department of Surgery, University of Illinois at Chicago, Chicago, Illinois
    • ,
  • Anthony Razzak, B.S.

      Affiliations

    • Department of Surgery, University of Illinois at Chicago, Chicago, Illinois
    • ,
  • Tricia A. Babcock, M.S.

      Affiliations

    • Department of Surgery, University of Illinois at Chicago, Chicago, Illinois
    • ,
  • W. Scott Helton, M.D., F.A.C.S.

      Affiliations

    • Departments of Surgery and Pharmacology, University of Illinois at Chicago, Chicago, Illinois
    • ,
  • N. Joseph Espat, M.D., M.S., F.A.C.S.

      Affiliations

    • Department of Surgery, Roger Williams Medical Center, Providence, Rhode Island
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at Surgical Oncology, Department of Surgery, Prior 4, Roger Williams Medical Center, 825 Chalkstone Avenue, Providence, RI 02908

Received 30 July 2007

References 

  1. Kromhout D, Bosschieter EB, de Lezenne CC. The inverse relation between fish consumption and 20-year mortality from coronary heart disease. N Engl J Med. 1985;312:1205
  2. Kremer JM, Robinson DR. Studies of dietary supplementation with omega-3 fatty acids in patients with rheumatoid arthritis. World Rev Nutr Diet. 1991;66:367
  3. Beck SA, Smith KL, Tisdale MJ. Anticachectic and antitumor effect of eicosapentaenoic acid and its effect on protein turnover. Cancer Res. 1991;51:6089
  4. Babcock TA, Helton WS, Hong D, et al. Omega-3 fatty acid lipid emulsion reduces LPS-stimulated macrophage TNF-alpha production. Surg Infect (Larchmt). 2002;3:145
  5. Lo CJ, Chiu KC, Fu M, et al. Fish oil decreases macrophage tumor necrosis factor gene transcription by altering the NFkappaB activity. J Surg Res. 1999;82:216
  6. Babcock TA, Novak T, Ong E, et al. Modulation of lipopolysaccharide-stimulated macrophage tumor necrosis factor-alpha production by omega-3 fatty acid is associated with differential cyclooxygenase-2 protein expression and is independent of interleukin-10. J Surg Res. 2002;107:135
  7. Bagga D, Wang L, Farias-Eisner R, et al. Differential effects of prostaglandin derived from omega-6 and omega-3 polyunsaturated fatty acids on COX-2 expression and IL-6 secretion. Proc Natl Acad Sci USA. 2003;100:1751
  8. Novak TE, Babcock TA, Jho DH, et al. NF-kappa B inhibition by omega-3 fatty acids modulates LPS-stimulated macrophage TNF-alpha transcription. Am J Physiol Lung Cell Mol Physiol. 2003;284:L84
  9. Babcock TA, Kurland A, Helton WS, et al. Inhibition of activator protein-1 transcription factor activation by omega-3 fatty acid modulation of mitogen-activated protein kinase signaling kinases. J Parenter Enteral Nutr. 2003;27:176
  10. Marletta MA. Nitric oxide synthase structure and mechanism. J Biol Chem. 1993;268:12231
  11. Stuehr DJ, Nathan CF. Nitric oxide (A macrophage product responsible for cytostasis and respiratory inhibition in tumor target cells). J Exp Med. 1989;169:1543
  12. Nathan CF, Hibbs JB. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr Opin Immunol. 1991;3:65
  13. MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol. 1997;15:323
  14. Guzik TJ, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol. 2003;54:469
  15. Xie QW, Kashiwabara Y, Nathan C. Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J Biol Chem. 1994;269:4705
  16. Lowenstein CJ, Alley EW, Raval P, et al. Macrophage nitric oxide synthase gene: Two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Proc Natl Acad Sci USA. 1993;90:9730
  17. Eberhardt W, Pluss C, Hummel R, et al. Molecular mechanisms of inducible nitric oxide synthase gene expression by IL-1beta and cAMP in rat mesangial cells. J Immunol. 1998;160:4961
  18. Guan Z, Baier LD, Morrison AR. p38 mitogen-activated protein kinase down-regulates nitric oxide and up-regulates prostaglandin E2 biosynthesis stimulated by interleukin-1beta. J Biol Chem. 1997;272:8083
  19. Babcock TA, Helton WS, Hong D, et al. Omega-3 fatty acid lipid emulsion reduces LPS-stimulated macrophage TNF-alpha production. Surg Infect (Larchmt). 2002;3:145
  20. Nussler AK, Billiar TR. Inflammation, immunoregulation, and inducible nitric oxide synthase. J Leukoc Biol. 1993;54:171
  21. Moncada S, Higgs EA. The discovery of nitric oxide and its role in vascular biology. Br J Pharmacol. 2006;147(Suppl 1):S193
  22. McCartney-Francis N, Allen JB, Mizel DE, et al. Suppression of arthritis by an inhibitor of nitric oxide synthase. J Exp Med. 1993;178:749
  23. Petros A, Bennett D, Vallance P. Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shock. Lancet. 1991;338:1557
  24. Vodovotz Y, Lucia MS, Flanders KC, et al. Inducible nitric oxide synthase in tangle-bearing neurons of patients with Alzheimer's disease. J Exp Med. 1996;184:1425
  25. Ohata T, Fukuda K, Takahashi M, et al. Suppression of nitric oxide production in lipopolysaccharide-stimulated macrophage cells by omega 3 polyunsaturated fatty acids. Jpn J Cancer Res. 1997;88:234
  26. Lu CY, Penfield JG, Khair-el-Din TA, et al. Docosahexaenoic acid, a constituent of fetal and neonatal serum, inhibits nitric oxide production by murine macrophages stimulated by IFN gamma plus LPS, or by IFN gamma plus Listeria monocytogenes. J Reprod Immunol. 1998;38:31

PII: S0022-4804(07)02406-7

doi: 10.1016/j.jss.2007.12.758

Journal of Surgical Research
Volume 149, Issue 2 , Pages 296-302 , October 2008

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