The Effect of U-74389G on Liver Recovery After Acute Liver Ischemia-Reperfusion Injury in a Swine Model

Tsaroucha, Alexandra K.; Papalois, Apostolos; Vernadakis, Spyridon; Adamopoulos, Stavros; Papadopoulos, Konstantinos; Lambropoulou, Maria; Constadinidis, Theodoros; Kyriazi, Avgi; Papadopoulos, Nikolaos; Simopoulos, Constantinos

doi:10.1016/j.jss.2008.01.024

« Previous Next »Journal of Surgical Research
Volume 151, Issue 1 , Pages 10-14, January 2009

The Effect of U-74389G on Liver Recovery After Acute Liver Ischemia-Reperfusion Injury in a Swine Model

Alexandra K. Tsaroucha, M.D., Ph.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
- To whom correspondence and reprint requests should be addressed at 2nd Department of Surgery, Medical School, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Greece
Apostolos Papalois, Ph.D.
- Experimental–Research Center, Elpen Pharmaceuticals, Athens, Greece
Spyridon Vernadakis, M.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Stavros Adamopoulos, M.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Konstantinos Papadopoulos, M.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Maria Lambropoulou, M.D., Ph.D.
- Laboratory of Histology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Theodoros Constadinidis, M.D., Ph.D.
- Laboratory of Hygiene and Environmental Protection, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Avgi Kyriazi, M.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Nikolaos Papadopoulos, M.D., Ph.D.
- Laboratory of Histology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Constantinos Simopoulos, M.D., Ph.D.
- 2nd Department of Surgery and Department of Experimental Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece

Received 11 September 2007 published online 12 May 2008.

Background

The potential of U-74389G in attenuating liver damage after ischemia and reperfusion of the liver was studied in a swine model.

Materials and methods

Eighteen pigs, weighting 28–35 kg, were used in the study. The animals were divided into the following three experimental groups: Group A (control group): Ischemia time 30 min and reperfusion for 120 min (with tissue and blood sampling at both 60 min (A-60) and 120 min (A-120)); Group B: Ischemia time 30 min, U-74389G intraportal injection, and reperfusion for 60 min; and Group C: Ischemia time 30 min, U-74389G intraportal injection, and reperfusion for 120 min. The dose of U-74389G administered was 10 mg/kg animal body weight. Anesthesia was induced with propofol, pancuronium, and fentanyl. Surgery was performed through a midline laparotomy. The portal vein and the common hepatic artery were isolated and prepared for occlusion.

Results

Histopathological evaluation revealed a statistically significant difference in portal infiltration in the liver tissue between control group A-60 and group B (P = 0.01), and between control group A-120 and group C (P = 0.002). Hemodynamic and metabolic data in the control and therapy groups at 0, 30, 60, and 120 min were not statistically significantly different. Tissue malondialdehyde levels were statistically significantly different. Tumor necrosis factor-α values were statistically significantly different between groups A-60 and B but not between groups A-120 and C.

Conclusion

Based on the histological data and the reduction of the malondialdehyde and tumor necrosis factor-α levels, administration of U-74389G in ischemia-reperfusion injury of the liver in a swine model has potential in attenuating liver damage.

Key Words: lazaroid, U-74389G, liver, ischemia, reperfusion, swine, MDA, TNF-α