Role of Cardiopulmonary Bypass and Arrested Heart Status in the Early Cell Distribution after Intracoronary Infusion of Bone Marrow Stromal Cells

Song, Peng; Zhang, Hao; Lu, Ming-jie; Li, Jun; Liu, Xue-wen; Wei, Ying-jie; Hu, Sheng-shou

doi:10.1016/j.jss.2008.03.009

« Previous Next »Journal of Surgical Research
Volume 153, Issue 1 , Pages 66-70, 1 May 2009

Role of Cardiopulmonary Bypass and Arrested Heart Status in the Early Cell Distribution after Intracoronary Infusion of Bone Marrow Stromal Cells

Peng Song, M.D.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China
Hao Zhang, M.D., Ph.D.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China
- To whom correspondence and reprint requests should be addressed at Department of Surgery, Research Center for Cardiac Regenerative Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167A Beilishilu, Beijing 100037, P.R. China
Ming-jie Lu, M.D.
- Unit of Magnetic Resonance Imaging, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Jun Li, B.Sc.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China
Xue-wen Liu, M.Sc.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China
Ying-jie Wei, Ph.D.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China
Sheng-shou Hu, M.D.
- Research Center for Cardiac Regenerative Medicine, the Ministry of Health and Department of Surgery, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing, China

Received 26 December 2007 published online 12 May 2008.

Background

Systemic distribution of bone marrow stromal cells (BMSCs) after intracoronary infusion (ICI) and the role of cardiopulmonary bypass (CPB) in cell distribution still remain unclear. This study was designed o analyze the cell distribution after ICI in variations of heart status in a swine myocardial infarction model.

Materials and methods

After inducing a myocardial infarction, iron oxide labeled male cells (1 × 10⁸) were infused through the coronary artery of the beating swine heart in Group 1. In Group 2, CPB was set up and then the same volume of cells was infused after cardioplegic arrest. In Groups 3 and 4, the animals underwent either beating or arrested ICI with the same volume of saline. Three days later, cell distribution was assessed by T2* change with magnetic resonance imaging and sex-determining region on Y-chromosome with quantitive polymerase chain reaction.

Results

Only a few transplanted cells were localized in the heart and no difference was found between Groups 1 and 2. The majority of BMSCs would be trapped in extracardial organs, and more cells resided in the spleen in arrested heart status.

Conclusions

The majority of BMSCs transplanted by ICI would be entrapped by the extracardial organs. The arrested heart with CPB during ICI does not favor more cell retention in the injured myocardium. The optimal approach of delivery of BMSCs still needs further investigation.

Key Words: cell transplantation, myocardial infarction, bone marrow stromal cell, cardiopulmonary bypass