Protective Effects of a Heme Oxygenase-1-Secreting Lactococcus lactis on Mucosal Injury Induced by Hemorrhagic Shock in Rats

Pang, Qing-feng; Ji, Yong; Bermúdez-Humarán, Luis G.; Zhou, Qiao-mei; Hu, Gang; Zeng, Yinming

doi:10.1016/j.jss.2008.03.042

« Previous Next »Journal of Surgical Research
Volume 153, Issue 1 , Pages 39-45, 1 May 2009

Protective Effects of a Heme Oxygenase-1-Secreting Lactococcus lactis on Mucosal Injury Induced by Hemorrhagic Shock in Rats

Qing-feng Pang, Ph.D.
- Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China
Yong Ji, Ph.D.
- Jiangsu Province Institute of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, People's Republic of China
Luis G. Bermúdez-Humarán, Ph.D.
- INRA, Jouy-en-Josas, France
Qiao-mei Zhou, M.D.
- Jiangsu Province Institute of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, People's Republic of China
Gang Hu, Ph.D.
- Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China
Yinming Zeng, Ph.D.
- Jiangsu Province Institute of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, People's Republic of China
- To whom correspondence and reprint requests should be addressed at Jiangsu Province institute of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, People's Republic of China, 221002

Received 20 December 2007 published online 12 May 2008.

Aim

To investigate the protective effects of a heme oxygenase-1 (HO-1)-secreting Lactococcus lactis (LL-HO-1) on mucosal injury induced by hemorrhagic shock in rats.

Methods

The ability of recombinant LL-HO-1 to secrete biological active HO-1 in the rat intestine was determined in situ after 3 d of daily intragastric administration. The therapeutic potential of LL-HO-1 strain was then evaluated on mucosal injury induced by hemorrhagic shock in rats. After successful resuscitation, mean arterial blood pressure was recorded at 5, 10, 20, and 30 min. One hour after resuscitation, the ileum was harvested for evaluation of mucosal injury by blinded microscopic inflammatory score (Chiu's grade 0–5), myeloperoxidase activity, bacterial translocation, and by the secretion of pro- and anti-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-10, respectively).

Results

Intragastric administration of HO-1-secreting L. lactis strain led to bioactive delivery of HO-1 at intestinal mucosa and significantly enhanced mean arterial blood pressure and interleukin-10 levels. Moreover, intragastric administration of LL-HO-1 significantly decreased Chiu's score, myeloperoxidase activity, bacterial translocation, and tumor necrosis factor-alpha levels when compared with rats treated with the wild-type strain. The protective effect of recombinant LL-HO-1 could be abolished by co-administration of a HO-1 inhibitor, the zinc protoporphyrin-IX.