Detection of Elevated Proteins in Peritoneal Dissemination of Gastric Cancer by Analyzing Mass Spectra Data of Serum Proteins

Kojima, Taiki; Yoshikawa, Kazuhiro; Saga, Shinsuke; Yamada, Tomohiro; Kure, Shigehiro; Matsui, Takanori; Uemura, Takanori; Fujimitsu, Yasunobu; Sakakibara, Masatoshi; Kodera, Yasuhiro; Kojima, Hiroshi

doi:10.1016/j.jss.2008.07.024

« Previous Next »Journal of Surgical Research
Volume 155, Issue 1 , Pages 13-17, July 2009

Detection of Elevated Proteins in Peritoneal Dissemination of Gastric Cancer by Analyzing Mass Spectra Data of Serum Proteins

Taiki Kojima, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan
- To whom correspondence and reprint requests should be addressed at the Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Kuriyado 18, Kakemachi, Okazaki-shi, Aichi-ken 444-0011, Japan.
Kazuhiro Yoshikawa, M.D.
- Research Complex for the Medicine Frontiers, Aichi Medical University, Aichi, Japan
Shinsuke Saga, M.D.
- Department of Pathology, Aichi Medical University, Aichi, Japan
Tomohiro Yamada, M.D.
- Research Complex for the Medicine Frontiers, Aichi Medical University, Aichi, Japan
Shigehiro Kure, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan
Takanori Matsui, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan
Takanori Uemura, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan
Yasunobu Fujimitsu, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan
Masatoshi Sakakibara, M.D.
- Department of Gastroenterological Medicine, Aichi Cancer Center Aichi Hospital, Aichi, Japan
Yasuhiro Kodera, M.D.
- Department of Surgery II, Nagoya University Graduate School of Medicine, Aichi, Japan
Hiroshi Kojima, M.D.
- Department of Gastroenterological Surgery, Aichi Cancer Center Aichi Hospital, Aichi, Japan

Received 23 February 2008 published online 29 August 2008.

Background

Recently, several authors reported on the Protein Chip approach to analyze serum. They used SELDI-TOF-MS (surface enhanced laser desorption/ionization-time of flight-mass spectrometry) to identify patients with cancers of various origins in a highly sensitive and specific manner. In the current study, a similar approach was employed to analyze the serum of patients with various stages of gastric cancer.

Methods

Control serum specimens from patients with gastritis (n = 19) and those with gastric cancer (Stage I: n = 6, Stage II or III: n = 6, Stage IV: n = 6, total: n = 18) were collected and analyzed by the Protein Chip biomarker system (Bio-Rad, Japan), a platform for SELDI-TOF-MS, and protein profiles were obtained and compared. The cation exchange chip (CM10) and the anion exchange chip (Q10) were used for processing before TOF-MS.

Results

nine proteins were significantly over-expressed (P < 0.05, Student t-test) in patients with gastric cancer compared to patients with gastritis. Among them, four protein masses with 2929 m/z, 3293 m/z, 3371 m/z, and 4213 m/z were found to be differentially expressed solely in patients suffering from peritoneal dissemination. All peaks were processed on CM10 chips. Employing one data mining method, CART (classification and regression trees), gastric cancer patients with peritoneal dissemination were successfully separated from those who had no peritoneal seeding.

Conclusion

A validation study with a larger number of samples is mandatory; however, the detected peaks here might be candidates for biomarkers of peritoneal dissemination and/or gastric cancer. Moreover, further analysis of these four proteins might be helpful in revealing the mechanism of peritoneal dissemination, which at present has no cure.

Key Words: gastric cancer, peritoneal dissemination, SELDI-TOF-MS, biomarker, CART