Journal of Surgical Research
Volume 157, Issue 1 , Pages 14-20 , November 2009

Cell Suspensions of Autologous Keratinocytes or Autologous Fibroblasts Accelerate the Healing of Full Thickness Skin Wounds in a Diabetic Porcine Wound Healing Model

  • Patrik Velander, M.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • Department of Clinical Science, Malmoe, Lund University, Malmoe, Sweden
    • Division of Surgery, Malmoe University Hospital, Malmoe, Sweden
    • ,
  • Christoph Theopold, M.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • ,
  • Oliver Bleiziffer, M.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • ,
  • Juri Bergmann, M.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • ,
  • Henry Svensson, M.D., Ph.D.

      Affiliations

    • Department of Clinical Science, Malmoe, Lund University, Malmoe, Sweden
    • Division of Surgery, Malmoe University Hospital, Malmoe, Sweden
    • ,
  • Yao Feng, Ph.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • ,
  • Elof Eriksson, M.D., Ph.D.

      Affiliations

    • Laboratory of Tissue Repair and Gene Transfer, Harvard Medical School, Division of Plastic Surgery, Brigham and Woman's Hospital, Boston, Massachusetts
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at Division of Plastic Surgery, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115.

Received 14 February 2008

References 

  1. Velander P, Theopold C, Hirsch T, et al. Impaired wound healing in an acute diabetic pig model and the effects of local hyperglycemia. Wound Repair Regen. 2008;16:288
  2. Svensjo T, Pomahac B, Yao F, et al. Accelerated healing of full-thickness skin wounds in a wet environment. Plast Reconstr Surg. 2000;106:602;discussion 613
  3. Svensjo T, Yao F, Pomahac B, et al. Autologous keratinocyte suspensions accelerate epidermal wound healing in pigs. J Surg Res. 2001;99:211
  4. Loots MA, Lamme EN, Mekkes JR, et al. Cultured fibroblasts from chronic diabetic wounds on the lower extremity (non-insulin-dependent diabetes mellitus) show disturbed proliferation. Arch Dermatol Res. 1999;291:93
  5. Svensjo T, Yao F, Pomahac B, et al. Cultured autologous fibroblasts augment epidermal repair. Transplantation. 2002;73:1033
  6. Vogt PM, Thompson S, Andree C, et al. Genetically modified keratinocytes transplanted to wounds reconstitute the epidermis. Proc Natl Acad Sci USA. 1994;91:9307
  7. Steed DL. Clinical evaluation of recombinant human platelet-derived growth factor for the treatment of lower extremity ulcers. Plast Reconstr Surg. 2006;117(7 Suppl):143S; discussion 150S
  8. Han SK, Kim DW, Jeong SH, et al. Potential use of blood bank platelet concentrates to accelerate wound healing of diabetic ulcers. Ann Plast Surg. 2007;59:532
  9. Schaffer M, Bongartz M, Fischer S, et al. Nitric oxide restores impaired healing in normoglycemic diabetic rats. J Wound Care. 2007;16:311
  10. Dechert TA, Ducale AE, Ward SI, et al. Hyaluronan in human acute and chronic dermal wounds. Wound Rep Regen. 2006;14:252
  11. Sweitzer SM, Fann SA, Borg TK, et al. What is the future of diabetic wound care?. Diabetes Educ. 2006;32:197
  12. Bell RH, Hye RJ. Animal models of diabetes mellitus: Physiology and pathology. J Surg Res. 1983;35:433
  13. Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabet Med. 2006;23:594
  14. Greenhalgh DG. Wound healing and diabetes mellitus. Clin Plast Surg. 2003;30:37
  15. Vranckx JJ, Hoeller D, Velander PE, et al. Cell suspension cultures of allogenic keratinocytes are efficient carriers for ex vivo gene transfer and accelerate the healing of full-thickness skin wounds by overexpression of human epidermal growth factor. Wound Rep Regen. 2007;15:657
  16. Bitar MS. Insulin and glucocorticoid-dependent suppression of the IGF-I system in diabetic wounds. Surgery. 2000;127:687
  17. Bitar MS, Labbad ZN. Transforming growth factor-beta and insulin-like growth factor-I in relation to diabetes-induced impairment of wound healing. J Surg Res. 1996;61:113
  18. Jeffcoate WJ, Price P, Harding KG. Wound healing and treatments for people with diabetic foot ulcers. Diabetes Metab Res Rev. 2004;20(Suppl 1):S78
  19. Kamal K, Powell RJ, Sumpio BE. The pathobiology of diabetes mellitus: implications for surgeons. J Am Coll Surg. 1996;183:271
  20. Komesu MC, Tanga MB, Buttros KR, et al. Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology. 2004;11:63
  21. Yuan S, Liu Y, Zhu L. Vascular complications of diabetes mellitus. Clin Exp Pharmacol Physiol. 1999;26:977
  22. Spravchikov N, Sizyakov G, Gartsbein M, et al. Glucose effects on skin keratinocytes: implications for diabetes skin complications. Diabetes. 2001;50:1627

PII: S0022-4804(08)00644-6

doi: 10.1016/j.jss.2008.10.001

Journal of Surgical Research
Volume 157, Issue 1 , Pages 14-20 , November 2009

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