Low-Density Lipoproteins Oxidized After Intestinal Ischemia/Reperfusion in Rats

Tekin, Ishak Ozel; Sipahi, Emine Yilmaz; Comert, Mustafa; Acikgoz, Serefden; Yurdakan, Gamze

doi:10.1016/j.jss.2008.11.006

« Previous Next »Journal of Surgical Research
Volume 157, Issue 1 , Pages e47-e54, November 2009

Low-Density Lipoproteins Oxidized After Intestinal Ischemia/Reperfusion in Rats

Ishak Ozel Tekin, M.D.
- Department of Immunology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey
Emine Yilmaz Sipahi, M.D.
- Department of Pharmacology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey
- To whom correspondence and reprint requests should be addressed at Department of Pharmacology, Zonguldak Karaelmas Universitesi, Tip Fakultesi Egitim Bloklari Farmakoloji A.D. 67600 Kozlu, Zonguldak, Turkey.
Mustafa Comert, M.D.
- Department of Surgery, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey
Serefden Acikgoz, M.D.
- Department of Biochemistry, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey
Gamze Yurdakan, M.D.
- Department of Pathology, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak, Turkey

Received 13 August 2008 published online 09 December 2008.

Background

Intestinal ischemia/reperfusion (I/R) is a complex phenomenon causing destruction of both local and remote tissues, as well as multiple-organ failure. We investigated the role of lipid peroxidation in damage to intestinal, liver, and lung tissues in this pathology.

Materials and Methods

The superior mesenteric artery was blocked for 30 min followed by 24 h of reperfusion. Tissues were removed and the presence of oxidized LDL, the activities of the superoxide dismutase enzyme, malondialdehyde levels, and inducible nitric oxide synthase expression were each evaluated in the intestinal, liver, and lung tissues.

Results

While there was no staining in the control group tissues, ischemia/reperfusion resulted in positive oxidized LDL staining in all of the I/R test group tissue samples. Inducible nitric oxide synthase expression was significantly increased in the ischemia/reperfusion group tissues. Compared with those of the control group rats, the ischemia/reperfusion group tissues showed significantly higher malondialdehyde levels and lower superoxide dismutase activities.

Conclusions

This study demonstrated for the first time that oxidized LDL accumulated in the terminal ileum, liver, and lung tissues after intestinal ischemia/reperfusion. This occurrence (or the presence of oxidized LDL) may be an indicator of ongoing oxidative stress and enhanced lipid peroxidation. Augmentation of inducible nitric oxide synthase expression may play a role in progression of inflammation and LDL oxidation. These data support the hypothesis that cellular oxidative stress is a critical step in reperfusion-mediated injury in both the intestine and end organs, and that antioxidant strategies may provide organ protection in patients with reperfusion injury, at least through affecting interaction with free radicals, nitric oxide, and oxidized LDL.

Key Words: lipid peroxidation, oxidized low-density lipoproteins, intestinal ischemia/reperfusion