Effects of Bone Marrow and Hepatocyte Transplantation on Liver Injury

Zhang, Biao; Inagaki, Mitsuhiro; Jiang, Bojian; Miyakoshi, Masaaki; Arikura, Jun; Ogawa, Katsuhiro; Kasai, Shinichi

doi:10.1016/j.jss.2008.12.013

« Previous Next »Journal of Surgical Research
Volume 157, Issue 1 , Pages 71-80, November 2009

Effects of Bone Marrow and Hepatocyte Transplantation on Liver Injury

Biao Zhang, M.D.
- Department of Surgery, Asahikawa Medical College, Japan
- Present address: Department of Sururey, No.3 People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai 201900, China.
Mitsuhiro Inagaki, M.D.
- Department of Surgery, Asahikawa Medical College, Japan
- To whom correspondence and reprint requests should be addressed at Department of Surgery, Asahikawa Medical College, Midorigaoka-Higashi 2-1-1-1, Asahikawa, 078-8510 Japan.
Bojian Jiang, M.D.
- Department of Surgery, Asahikawa Medical College, Japan
- Present address: Department of Sururey, No.3 People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai 201900, China.
Masaaki Miyakoshi, D.D.S.
- Department of Pathology, Asahikawa Medical College, Japan
Jun Arikura, M.D.
- Department of Surgery, Asahikawa Medical College, Japan
Katsuhiro Ogawa, M.D.
- Department of Pathology, Asahikawa Medical College, Japan
Shinichi Kasai, M.D.
- Department of Surgery, Asahikawa Medical College, Japan

Received 1 September 2008 published online 07 January 2009.

Background

The therapeutic effects of bone marrow and hepatocyte transplantation were investigated regarding the treatment of retrorsine-partial hepatectomy-induced liver injury.

Methods

Analbuminemic F344alb rats were given two doses of retrorsine 2 wk apart, followed 4 wk later by transplantation with F344 rat bone marrow cells or hepatocytes immediately after a two-thirds hepatectomy. The survival rate, liver regeneration rate, liver functions, albumin-positive hepatocytes, and normal albumin gene sequences in the liver and serum albumin levels were investigated in the recipients.

Results

Although 65% retrorsine/partial hepatectomy-treated F344alb died between 1 and 11 d after the partial hepatectomy, only 27.5% of the animals died following bone marrow transplantation, and 50% with hepatocyte transplantation. Both bone marrow and hepatocyte transplantation ameliorated acute liver injury after a partial hepatectomy. Bone marrow transplantation yielded a very small increase in the number of albumin-positive hepatocytes in the liver, while hepatocyte transplantation resulted in massive replacement of the liver tissues by the donor hepatocytes associated with an elevation of serum albumin after an extended time.