Journal of Surgical Research
Volume 159, Issue 1 , Pages 565-571 , March 2010

Cyclic AMP Inhibits IL-1β Plus IFNγ-Induced NF-κB Translocation in Hepatocytes by a PKA Independent Mechanism

  • Guiying Hong, M.D.

      Affiliations

    • Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvinia
    • ,
  • Baochun Zhang, Ph.D.

      Affiliations

    • Department of Surgery, University of Louisville Health Science Center, Louisville, Kentucky
    • Corresponding Author InformationTo whom correspondence and reprint requests should be addressed at Department of Surgery, University of Louisville Health Science Center, MDR Building, Suite 317, 511 S. Floyd Street, Louisville, KY 40202.
    • ,
  • Brian G. Harbrecht, M.D.

      Affiliations

    • Department of Surgery, University of Louisville Health Science Center, Louisville, Kentucky

Received 25 June 2008

References 

  1. Barnes PJ, Karin M. Nuclear factor-κB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 1997;336:1066–1071
  2. Siebenlist U, Franzoso G, Brown K. Structure, regulation and function of NF-κB. Annu Rev Cell Biol. 1994;10:405–455
  3. Li Q, Verma IM. NF-κB regulation in the immune system. Nat Rev Immunol. 2002;2:725–734
  4. Pomerantz JL, Baltimore D. Two pathways to NF-κB. Mol Cell. 2002;10:693–695
  5. Chen Z, Hagler J, Palombella VJ, Melandri F, Scherer D, Ballard D, et al. Signal-induced site-specific phosphorylation targets IκB alpha to the ubiquitin-proteasome pathway. Genes Dev. 1995;9:1586–1597
  6. Dobashi K, Asayama K, Shirahata A. Differential effects of cyclic AMP on induction of nitric oxide synthase in 3T3-L1 cells and brown adipocytes. Free Radic Biol Med. 2003;35:94–101
  7. Kamthong PJ, Wu M. Inhibitor of nuclear factor-κB induction by cAMP antagonizes interleukin-1-induced human macrophage-colony-stimulating-factor expression. Biochem J. 2001;356:525–530
  8. Chio CC, Chang YH, Hsu YW, Chi KH, Lin WW. PKA-dependent activation of PKC, p38 MAPK and IKK in macrophage: implication in the induction of inducible nitric oxide synthase and interleukin-6 by dibutyryl cAMP. Cell Signal. 2004;16:565–575
  9. Woo MS, Jang PG, Park JS, Kim WK, Joh TH, Kim HS. Selective modulation of lipopolysaccharide-stimulated cytokine expression and mitogen-activated protein kinase pathways by dibutyryl-cAMP in BV2 microglial cells. Brain Res Mol Brain Res. 2003;113:86–96
  10. Harbrecht BG, Taylor BS, Xu Z, Ramalakshmi S, Ganster RW, Geller DA. cAMP inhibits inducible nitric oxide synthase expression and NF-κB-binding activity in cultured rat hepatocytes. J Surg Res. 2001;99:258–264
  11. Taylor SS, Buechler JA, Yonemoto W. cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Annu Rev Biochem. 1990;59:971–1005
  12. Traenckner EB, Pahl HL, Henkel T, Schmidt KN, Wilk S, Baeuerle PA. Phosphorylation of human IκB-α on serines 32 and 36 controls IκB-alpha proteolysis and NF-κB activation in response to diverse stimuli. Embo J. 1995;14:2876–2883
  13. Schreiber E, Matthias P, Muller MM, Schaffner W. Rapid detection of octamer binding proteins with ‘mini-extracts’, prepared from a small number of cells. Nucleic Acids Res. 1989;17:6419
  14. Zhang B, Liu S, Perpetua MD, Walker WH, Harbrecht BG. Cytokines increase CRE binding but decrease CRE-mediated reporter activity in rat hepatocytes by increasing c-Jun. Hepatology. 2004;39:1343–1352
  15. Zhang B, Perpetua M, Fulmer M, Harbrecht BG. JNK signaling involved in the effects of cyclic AMP on IL-1β plus IFNγ-induced inducible nitric oxide synthase expression in hepatocytes. Cell Signal. 2004;16:837–846
  16. Williams JA, Shacter E. Regulation of macrophage cytokine production by prostaglandin E2. Distinct roles of cyclooxygenase-1 and -2. J Biol Chem. 1997;272:25693–25699
  17. Zhang Y, Lin JX, Vilcek J. Synthesis of interleukin 6 (interferon-β 2/B cell stimulatory factor 2) in human fibroblasts is triggered by an increase in intracellular cyclic AMP. J Biol Chem. 1988;263:6177–6182
  18. Kunz D, Muhl H, Walker G, Pfeilschifter J. Two distinct signaling pathways trigger the expression of inducible nitric oxide synthase in rat renal mesangial cells. Proc Natl Acad Sci U S A. 1994;91:5387–5391
  19. Sowa G, Przewlocki R. cAMP analogues and cholera toxin stimulate the accumulation of nitrite in rat peritoneal macrophage cultures. Eur J Pharmacol. 1994;266:125–129
  20. Imai T, Hirata Y, Kanno K, Marumo F. Induction of nitric oxide synthase by cyclic AMP in rat vascular smooth muscle cells. J Clin Invest. 1994;93:543–549
  21. Bos JL. Epac: a new cAMP target and new avenues in cAMP research. Nat Rev Mol Cell Biol. 2003;4:733–738
  22. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature. 1997;388:548–554
  23. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, necessary for IκB phosphorylation and NF-κB activation. Cell. 1997;91:243–252

PII: S0022-4804(08)01577-1

doi: 10.1016/j.jss.2008.12.018

Journal of Surgical Research
Volume 159, Issue 1 , Pages 565-571 , March 2010

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