Development and Analysis of a Collagen-Based Hemostatic Adhesive

Baik, Seung Hyuk; Kim, Jae Hyung; Cho, Hyun Hee; Park, Si-Nae; Kim, Yu Seun; Suh, Hwal

doi:10.1016/j.jss.2010.08.004

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Volume 164, Issue 2 , Pages e221-e228, December 2010

Development and Analysis of a Collagen-Based Hemostatic Adhesive

Seung Hyuk Baik, M.D., Ph.D.
- Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea
- Program of Nano Science and Technology, Graduate School, Yonsei University, Seoul, Korea
- Department of Surgery, Severance Hospital, Yonsei University, Seoul, Korea
Jae Hyung Kim, M.S.
- Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea
- Program of Nano Science and Technology, Graduate School, Yonsei University, Seoul, Korea
Hyun Hee Cho, M.S.
- Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea
- Program of Nano Science and Technology, Graduate School, Yonsei University, Seoul, Korea
Si-Nae Park, Ph.D
- Dalim-Tissen Co., Ltd, Seoul, Korea
Yu Seun Kim, M.D., Ph.D.
- Program of Nano Science and Technology, Graduate School, Yonsei University, Seoul, Korea
- Department of Surgery, Severance Hospital, Yonsei University, Seoul, Korea
Hwal Suh, D.D.S, Ph.D.
- Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea
- Program of Nano Science and Technology, Graduate School, Yonsei University, Seoul, Korea
- Department of Medical Engineering, Research Center for Nano Medical engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- To whom correspondence and reprint requests should be addressed at Laboratory of Tissue Regenerative Medicine, Department of Medical Engineering, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-ku, 120-752, Seoul, Korea.

Received 10 May 2010 published online 01 September 2010.

Background

Commercially available hemostatic-adhesive has the risk of disease transmission because it is derived from a plasma component. The purpose of this study is to manufacture a new hemostatic adhesive and evaluate its performances.

Methods

Two atelocollagen-based hemostatic adhesives were produced and named as Bleestop A (2% esterified atelocollagen in 75% ethanol + 1% CaCl₂ + 0.71% DOPA + 0.1% tranexamic acid in DW) and Bleestop B (2% esterified atelocollagen in DW + 1% CaCl₂ + 0.71% DOPA + 0.1% aminocaproic acid in DW). These are compared with the negative control group (no adhesives used group) and the positive control group (Tissucol Duo Quick group). The adult male Sprague-Dawley rat model was adopted and the hemostatic-adhesion activities of each group were assessed by the adhesion strength test and the morphologic features of adhesion. The liver tissues were used. Histologic assessment of adhesion was accessed using light microscopy.

Results

Bleeding was controlled immediately after application of Bleestop A and partial adhesion was observed after 30 s. More than 95% area of the resected surface attached after 1 min. In Bleestop B, partial adhesion was observed after 30 s. More than 95% area of the resected surface was attached after 45 s. Histologic evaluation showed that Bleestop A and B mediated organized adhesion rapidly between both resected tissue surfaces. The adhesion strength of Bleestop A and B was better than the negative control group and showed same adhesion strength as the positive control group after 3 min.