Activation of Cholinergic Anti-Inflammatory Pathway Contributes to the Protective Effects of 100% Oxygen Inhalation on Zymosan-Induced Generalized Inflammation in Mice

Zhang, Zishen; Bai, Xiaoguang; Du, Kejun; Huang, Yi; Wang, Wenyong; Zhao, Yiling; Pei, Yuping; Mu, Jinglan; Han, Han; Hu, Sheng; Li, Shuzhi; Dong, Hailong; Lu, Yan; Hou, Lichao; Xiong, Lize

doi:10.1016/j.jss.2011.10.037

« Previous Next »Journal of Surgical Research
Volume 174, Issue 2 , Pages e75-e83, 15 May 2012

Activation of Cholinergic Anti-Inflammatory Pathway Contributes to the Protective Effects of 100% Oxygen Inhalation on Zymosan-Induced Generalized Inflammation in Mice

Zishen Zhang, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- These authors contributed equally to this work.
Xiaoguang Bai, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- These authors contributed equally to this work.
Kejun Du, Ph.D., M.D.
- Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- These authors contributed equally to this work.
Yi Huang, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- These authors contributed equally to this work.
Wenyong Wang, M.D.
- Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Yiling Zhao, M.D.
- Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Yuping Pei, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Jinglan Mu, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Han Han, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Sheng Hu, Ph.D., M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Shuzhi Li, M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Hailong Dong, Ph.D., M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Yan Lu, Ph.D., M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
Lichao Hou, Ph.D., M.D.
- Surgical ICU, Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- To whom correspondence and reprint requests should be addressed at Surgical ICU, Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China.
Lize Xiong, Ph.D., M.D.
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China
- Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, P. R. China.

Received 2 June 2011; accepted 26 October 2011. published online 23 November 2011.

Background

The 100% oxygen inhalation has been demonstrated to have a protective effect on mice with zymosan-induced generalized inflammation. However, the underlying mechanism is largely unknown. The present study was designed to explore the role of the cholinergic anti-inflammatory pathway in this animal model.

Methods

Oxygen inhalation was given to mice at 4 and 12 h after zymosan injection. One group of mice underwent vagotomy 7 d before zymosan injection. The other two groups of mice either received nicotinic acetylcholine receptor (nAChR) antagonist mecamylamine, or α7 nicotinic acetylcholine receptor (α7nAChR) antagonist methyllycaconitine 30 min before oxygen was given.

Results

The 100% oxygen treatment significantly decreased the serum level of TNF-α and increased the serum level of IL-10. The pathologic changes of the heart, lung, liver, and kidney were attenuated, as well as the dysfunction of liver and kidney. The 7-d survival rate of zymosan-challenged mice was also improved. Conversely, all these protective effects caused by pure oxygen treatment were abolished in those animals that received anti-cholinergic treatments.

Conclusions

The cholinergic anti-inflammatory pathway may be involved in the 100% oxygen protective mechanism against zymosan-induced generalized inflammation in mice.

Key Words: zymosan, systemic inflammation, cholinergic anti-inflammatory pathway, inflammatory cytokine, 100% oxygen