Background
Hydrogen has been considered as a novel antioxidant that prevents injuries resulted
from ischemia-reperfusion (I/R) injury in various tissues. The study was designed
to determine the effect of hydrogen-rich saline on the smooth muscle contractile response
to KCl, and on epithelial proliferation and apoptosis of intestine subjected to I/R.
Methods
Intestinal I/R injury was induced in Sprague-Dawley rats using bulldog clamps in superior
mesenteric artery by 45 min ischemia followed by 1 h reperfusion. Rats were divided
randomly into four groups: sham-operated, I/R, I/R plus saline treatment, and I/R
plus hydrogen-rich saline treatment groups. Hydrogen-rich saline (>0.6 mM, 6 mL/kg)
or saline (6 mL/kg) was administered, respectively, via tail vein 30 min prior to reperfusion. Following reperfusion, segments of terminal
jejunum were rapidly taken and transferred into isolated organ bath and responses
to KCl were recorded. Samples of terminal jejunum were also taken for measuring malondialdehyde
and myeloperoxidase. Apoptosis in intestinal epithelium was determined with terminal
deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling
technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen
(PCNA) were detected with immunohistochemistry.
Results
Hydrogen-rich saline treatment significantly attenuated the severity of intestinal
I/R injury, with inhibiting of I/R-induced apoptosis, and promoting enterocytes proliferation.
Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration,
lipid oxidation, and ameliorated the decreased contractility response to KCl in the
intestine subjected to I/R.
Conclusions
These results suggest that hydrogen treatment has a protective effect against intestinal
contractile dysfunction and damage induced by intestinal I/R. This protective effect
is possibly due to its ability to inhibit I/R-induced oxidative stress, apoptosis,
and to promote epithelial cell proliferation.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Surgical ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury.Anesthesiology. 2001; 94: 1133
- Bacterial translocation in clinical intestinal transplantation.Transplantation. 2001; 71: 1414
- Pathophysiology of mesenteric ischemia/reperfusion: A review.Acta Cir Bras. 2005; 20: 336
- Lymphocytes and ischemia-reperfusion injury.Transplant Rev (Orlando). 2009; 23: 1
- Alterations of intestinal motor responsiveness in a model of mild mesenteric ischemia/reperfusion in rats.Life Sci. 2002; 71: 2025
- Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum.Life Sci. 2005; 76: 1575
- Inducible nitric oxide synthase mediates gut ischemia/reperfusion-induced ileus only after severe insults.J Surg Res. 2001; 97: 150
- Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa.Am J Physiol. 1998; 274: G270
- The hydrogen highway to reperfusion therapy.Nat Med. 2007; 13: 673
- Oxygen-derived free radicals in postischemic tissue injury.N Engl J Med. 1985; 312: 159
- Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury.Am J Transplant. 2008; 8: 2015
- Anti-inflammatory properties of molecular hydrogen: Investigation on parasite-induced liver inflammation.C R Acad Sci III. 2001; 324: 719
- Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice.Biochem Biophys Res Commun. 2008; 377: 1195
- Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury.Biochem Biophys Res Commun. 2008; 373: 30
- Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals.Nat Med. 2007; 13: 688
- Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats.Free Radic Res. 2009; 43: 478
- Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats.Biochem Biophys Res Commun. 2009; 381: 602
- Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model.Brain Res. 2009; 1256: 129
- Erythropoietin protects the intestine against ischemia/ reperfusion injury in rats.Mol Med. 2007; 13: 509
- Contractile function of the mechanically lengthened intestine.J Surg Res. 2006; 136: 8
- Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.Anal Biochem. 1979; 95: 351
- Basic fibroblast growth factor reduces the gut and liver morphologic and functional injuries after ischemia and reperfusion.J Trauma. 1997; 42: 1080
- Activation of immediate early gene, c-fos, and c-jun in the rat small intestine after ischemia/reperfusion.Transplantation. 2000; 69: 598
- Acid fibroblast growth factor reduces rat intestinal mucosal damage caused by ischemia-reperfusion insult.World J Gastroenterol. 2005; 11: 6477
- Reperfusion injury after intestinal ischemia.Crit Care Med. 1993; 21: 1376
- Ischemia-reperfusion injury of the intestine and protective strategies against injury.Dig Dis Sci. 2004; 49: 1359
- Free radicals and antioxidants in normal physiological functions and human disease.Int J Biochem Cell Biol. 2007; 39: 44
- Reactive nitrogen species in the chemical biology of inflammation.Arch Biochem Biophys. 2004; 423: 12
- Protective effects of a new stable, highly active SOD mimetic, M40401 in splanchnic artery occlusion and reperfusion.Br J Pharmacol. 2001; 132: 19
- Effect of deferoxamine and L-arginine treatment on lipid peroxidation in an intestinal ischemia-reperfusion model in rats.Acta Vet Hung. 2002; 50: 343
- Preconditioning with ethanol prevents postischemic leukocyte-endothelial cell adhesive interactions.Am J Physiol Heart Circ Physiol. 2002; 283: H1019
- Consumption of molecular hydrogen prevents the stress-induced impairments in hippocampus-dependent learning tasks during chronic physical restraint in mice.Neuropsychopharmacology. 2009; 34: 501
- Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice.Biochem Biophys Res Commun. 2008; 375: 346
- Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance.Nutr Res. 2008; 28: 137
- Glucagonlike peptide-2 analogue enhances intestinal mucosal mass and absorptive function after ischemia-reperfusion injury.J Pediatr Surg. 2000; 35: 1537
- Intestinal ischemia/reperfusion injury produces chronic abnormalities of absorptive function.Transplant Proc. 2002; 34: 984
Article info
Publication history
Published online: August 28, 2009
Received:
April 20,
2009
Identification
Copyright
© 2011 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
