Advertisement

Outcome of microdialysis sampling on liver surface and parenchyma

Published:September 11, 2015DOI:https://doi.org/10.1016/j.jss.2015.09.009

      Abstract

      Background

      To investigate whether surface microdialysis (μD) sampling in probes covered by a plastic film, as compared to noncovered and to intraparenchymatous probes, would increase the technique's sensitivity for pathophysiologic events occurring in a liver ischemia-reperfusion model. Placement of μD probes in the parenchyma of an organ, as is conventionally done, may cause adverse effects, e.g., bleeding, possibly influencing outcome.

      Methods

      A transient ischemia-reperfusion model of the liver was used in six anesthetized normoventilated pigs. μD probes were placed in the parenchyma and on the liver surface. Surface probes were either left uncovered or were covered by plastic film.

      Results

      Lactate and glucose levels were significantly higher in plastic film covered probes than in uncovered surface probes throughout the ischemic period. Glycerol levels were significantly higher in plastic film covered probes than in uncovered surface probes at 30 and 45 min into ischemia.

      Conclusions

      Covering the μD probe increases the sensibility of the μD–technique in monitoring an ischemic insult and reperfusion in the liver. These findings confirm that the principle of surface μD works, possibly replacing need of intraparenchymatous placement of μD probes. Surface μD seemingly allows, noninvasively from an organ's surface, via the extracellular compartment, assessment of intracellular metabolic events. The finding that covered surface μD probes allows detection of local metabolic changes earlier than do intraparenchymatous probes, merit further investigation focusing on μD probe design.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Journal of Surgical Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Isaksson B.
        • D'souza M.A.
        • Jersenius U.
        • et al.
        Continuous assessment of intrahepatic metabolism by microdialysis during and after portal triad clamping.
        J Surg Res. 2011; 169: 214
        • Aberg A.M.
        • Ahlström K.
        • Abrahamsson P.
        • et al.
        Ischaemic pre-conditioning means an increased adenosine metabolism with decreased glycolytic flow in ischaemic pig myocardium.
        Acta Anaesthesiol Scand. 2010; 54: 1257
        • Gillispie A.
        • Rooyackers O.
        • Wernerman J.
        • Nowak G.
        Effect of extended cold ischemia time on glucose metabolism in liver grafts: experimental study in pigs.
        J Hepatobiliary Pancreat Surg. 2007; 14: 183
        • Mehrabi A.
        • Golling M.
        • Busch C.
        • et al.
        Experimental monitoring of hepatic glucose, lactate, and glutamate metabolism by microdialysis during surgical preparation of the liver hilus.
        J Surg Res. 2002; 105: 128
        • Nowak G.
        • Ungerstedt J.
        • Wernerman J.
        • Ungerstedt U.
        • Ericzon B.
        Clinical experience in continuous graft monitoring with microdialysis early after liver transplantation.
        Br J Surg. 2002; 89: 1169
        • Nowak G.
        • Ungerstedt J.
        • Wernerman J.
        • Ungerstedt U.
        • Ericzon B.
        Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model.
        Liver Transpl. 2002; 8: 424
        • Nowak G.
        • Ungerstedt J.
        • Wernerson A.
        • Ungerstedt U.
        • Ericzon B.
        Hepatic cell membrane damage during cold preservation sensitizes liver grafts to rewarming injury.
        J Hepatobiliary Pancreat Surg. 2003; 10: 200
        • Ungerstedt J.
        • Nowak G.
        • Ungerstedt U.
        • Ericzon B.
        Microdialysis monitoring of porcine liver metabolism during warm ischemia with arterial and portal clamping.
        Liver Transpl. 2009; 15: 280
        • Wikström G.
        • Ronquist G.
        • Nilsson S.
        • Maripu E.
        • Waldenström A.
        Continuous monitoring of energy metabolites using microdialysis during myocardial ischaemia in the pig.
        Eur Heart J. 1995; 16: 339
        • Schoemaker M.
        • Andreis E.
        • Röper J.
        • et al.
        The SCGM1 System: subcutaneous continuous glucose monitoring based on microdialysis technique.
        Diabetes Technol Ther. 2003; 5: 599
        • Perera M.T.
        • Richards D.A.
        • Silva M.A.
        • et al.
        Comparison of energy metabolism in liver grafts from donors after circulatory death and donors after brain death during cold storage and reperfusion.
        Br J Surg. 2014; 101: 775
        • Abrahamsson P.
        • Johansson G.
        • Aberg A.M.
        • Haney M.
        • Winsö O.
        Optimised sample handling in association with use of the CMA 600 analyser.
        J Pharm Biomed Anal. 2008; 48: 940
        • Ungerstedt U.
        Microdialysis–principles and applications for studies in animals and man.
        J Intern Med. 1991; 230: 365
        • Stahl M.
        • Bouw R.
        • Jackson A.
        • Pay V.
        Human microdialysis.
        Curr Pharm Biotechnol. 2002; 3: 165
        • Abrahamsson P.
        • Winsö O.
        An assessment of calibration and performance of the microdialysis system.
        J Pharm Biomed Anal. 2005; 39: 730
        • Abrahamsson P.
        • Aberg A.M.
        • Johansson G.
        • Winsö O.
        • Waldenström A.
        • Haney M.
        Detection of myocardial ischaemia using surface microdialysis on the beating heart.
        Clin Physiol Funct Imaging. 2011; 31: 175
        • Abrahamsson P.
        • Aberg A.M.
        • Winsö O.
        • Johansson G.
        • Haney M.
        • Blind P.J.
        Surface microdialysis sampling: a new approach described in a liver ischaemia model.
        Clin Physiol Funct Imaging. 2012; 32: 99
        • Waldenström A.
        • Haney M.
        • Biber B.
        • et al.
        Ischaemic preconditioning relates to decreasing levels of extracellular adenosine that may be energy-metabolically useful in myocardium at risk; an experimental study in the pig.
        Acta Physiol (Oxf). 2009;