Background
Some of the postulated molecular mechanisms of sepsis progression are linked with
the imbalance between reactive oxygen species (ROS) production and its degradation
by cellular antioxidant pathways. Some studies have correlated plasma oxidative stress,
inflammatory markers, and clinical markers of organ failure, but none performed this
in a systematic way, determining in situ oxidative and inflammatory markers and correlating these with markers of organ failure.
Materials and Methods
Rats subjected to cecal ligation and puncture (CLP) were treated with basic support
or antioxidants and killed 12 h after to determine thiobarbituric acid reactive species
(as an index of oxidative damage), superoxide dismutase (SOD), catalase (CAT), and
myeloperoxidase (MPO) (as an index of neutrophil infiltration) in the kidney and lung.
In addition, protein content in bronchoalveolar lavage fluid (as an index of lung
alveolo-capillary dysfunction) and plasma urea (as an index of kidney injury) were
measured at the same time.
Results
In the CLP group, we found a positive correlation between thiobarbituric acid reactive
species (TBARS) and markers of organ injury in lung and kidney. Oxidative damage is
correlated with an increase in SOD/CAT ratio only in the lung. In contrast, oxidative
damage is correlated with MPO activity in the kidney, but not lung, suggesting different
sources of oxidative damage depending on the analyzed organ. These reflect differences
on the effects of basic support and antioxidants on organ dysfunction after sepsis.
Conclusion
Despite the general occurrence of oxidative damage in different organs during sepsis
development and a positive correlation between oxidative markers and organ injury,
antioxidant effects seemed to depend not only on the diminution of oxidative damage
but also on its anti-inflammatory activity.
Key Words
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Article info
Publication history
Published online: September 10, 2009
Received:
June 25,
2009
Identification
Copyright
© 2011 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
