Background
Intestinal ischemia-reperfusion is a common medical event associated with both clinical
and experimental distant organ injury. In particular, the lung tissue appears to be
susceptible to injury resulting from systemic inflammatory mediator activation. Drotrecogin
α (activated) or recombinant human activated protein C has antithrombotic, anti-inflammatory,
and profibrinolytic properties. We hypothesized that APC infusion would decrease lung
inflammation and ameliorate lung injury resulting from intestinal ischemia-reperfusion
(IIR). A rat model of intestinal ischemia-reperfusion was used to test this hypothesis,
and several parameters of lung injury were measured in lung samples.
Material and Methods
Forty Wistar albino rats were divided into four groups: a sham-operated group (Sham),
an ischemic control group (IIR), an APC-infusion group (IIR'APC), and a normal saline-infusion
group (IIR'NS) (n = 10, each). A marker for lipid peroxidation, malondialdehyde (MDA), free radical
scavenger glutathione peroxidase (GSH-Px), an index of polymorphonuclear neutrophils,
myeloperoxidase (MPO) activity, and lung polymorphonuclear leukocytes (PMNL) were
investigated in the lung tissue samples.
Results
MDA and MPO levels, and lung PMNL sequestration were decreased, but GSH-Px levels
were increased in APC treated group versus IIR group. MDA levels were decreased and GSH-Px levels were increased in NS treated
group versus IIR group. MPO levels and lung PMNL counts were similar across the IIR and IIR'NS
groups.
Conclusions
This study documents that APC attenuates acute lung injury in intestinal ischemia-reperfusion. NS
infusion had also some favorable effects regarding MDA and MPO.
Key Words
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Article info
Publication history
Published online: December 31, 2009
Received:
August 1,
2009
Identification
Copyright
© 2011 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
