Background
Hydrogen has been proven to be a novel antioxidant through its selectively reducing
of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich
saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats.
Materials and Methods
Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline was administered
through intraperitoneal (i.p.) injection during exposure to hyperoxia (10 mL/Kg),
respectively.
Results
Severity of HALI was assessed by the volume of pleural effusion, wet-to-dry weight
ratio (W/D), and histologic analysis. Apoptosis in lung cells was determined with
terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining.
The content of pro-inflammatory cytokine interleukin IL-1b and TNF-a in the lung tissues
were detected by enzyme-linked immunosorbent assay (ELISA). Hydrogen-rich saline treatment
provides protection against HALI by inhibiting lipid, DNA oxidation, and tissue edema.
Moreover, hydrogen-rich saline treatment could inhibit apoptosis and inflammation
while no significant reduction was observed in nitrogen-rich saline treated animals.
Conclusion
The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced
acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue.
Key Words
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References
- IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.Am J Respir Cell Mol Biol. 2009; 40: 314
- Oxygen toxicity in mouse lung: Pathways to cell death.Am J Respir Cell Mol Biol. 1998; 19: 573
- Bcl-2 family gene expression during severe hyperoxia-induced lung injury.Lab Invest. 2000; 80: 1845
- Mechanisms of cell death in hypoxia/reoxygenation injury.Oncogene. 1998; 17: 3341
- Targeted lung expression of interleukin-11 enhances murine tolerance of 100% oxygen and diminishes hyperoxia-induced DNA fragmentation.J Clin Invest. 1998; 101: 1970
- Hyperoxia-induced apoptosis and Fas/FasL expression in lung epithelial cells.Am J Physiol Lung Cell Mol Physiol. 2005; 289: L647
- The effects of hyperoxia exposure on lung function and pulmonary surfactant in a rat model of acute lung injury.Exp Lung Res. 2009; 35: 380
- Lung cell oxidant injury. Enhancement of polymorphonuclear leukocyte-mediated cytotoxicity in lung cells exposed to sustained in vitro hyperoxia.J Clin Invest. 1982; 70: 342
- Disruption of Nrf2 impairs the resolution of hyperoxia-induced acute lung injury and inflammation in mice.J Immunol. 2009; 182: 7264
- Recombinant human superoxide dismutase reduces lung injury caused by inhaled nitric oxide and hyperoxia.Am J Physiol. 1997; 272: L903
- Increased sensitivity of homozygous Sod2 mutant mice to oxygen toxicity.Free Radic Biol Med. 2002; 32: 175
- Overexpression of cellular glutathione peroxidase does not affect expression of plasma glutathione peroxidase or phospholipid hydroperoxide glutathione peroxidase in mice offered diets adequate or deficient in selenium.J Nutr. 1997; 127: 675
- Mice deficient in cellular glutathione peroxidase show increased vulnerability to malonate, 3-nitropropionic acid, and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine.J Neurosci. 2000; 20: 1
- Transgenic mice overexpressing peroxiredoxin 6 show increased resistance to lung injury in hyperoxia.Am J Respir Cell Mol Biol. 2006; 34: 481
- Lung injury and mortality with hyperoxia are increased in peroxiredoxin 6 gene-targeted mice.Free Radic Biol Med. 2004; 37: 1736
- Recombinant tumor necrosis factor/cachectin and interleukin 1 pretreatment decreases lung oxidized glutathione accumulation, lung injury, and mortality in rats exposed to hyperoxia.J Clin Invest. 1987; 79: 1868
- Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals.Nat Med. 2007; 13: 688
- Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model.Brain Res. 2009; 1256: 129
- Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress.Biochem Biophys Res Commun. 2007; 361: 670
- Hydrogen inhalation ameliorates oxidative stress in transplantation-induced intestinal graft injury.Am J Transplant. 2008; 8: 2015
- Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury.Biochem Biophys Res Commun. 2008; 373: 30
- Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats.Exp Biol Med (Maywood). 2009; 234: 1212
- Hydrogen-rich saline protects against intestinal ischemia/reperfusion injury in rats.Free Radic Res. 2009; 43: 478
- Hydrogen-rich saline ameliorates the severity of l-arginine-induced acute pancreatitis in rats.Biochem Biophys Res Commun. 2010; 393: 308
- Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate.Biochem Biophys Res Commun. 2009; 386: 11
- Anti-inflammatory properties of molecular hydrogen: investigation on parasite-induced liver inflammation.C R Acad Sci III. 2001; 324: 719
- Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats.Biochem Biophys Res Commun. 2009; 381: 602
- Hyperoxia-induced signal transduction pathways in pulmonary epithelial cells.Free Radic Biol Med. 2007; 42: 897
- Mechanisms of cell injury and death in hyperoxia: Role of cytokines and Bcl-2 family proteins.Am J Respir Cell Mol Biol. 2000; 22: 517
- Inhibition of TNFα in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells.Respir Res. 2005; 6: 10
Article info
Publication history
Published online: October 18, 2010
Received:
August 21,
2010
Identification
Copyright
© 2011 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
