Background
Of solid organ transplantations, pancreas transplantation is associated with the highest
incidence of pancreatic fibrosis in the early post-transplantation period. Activated
pancreatic stellate cells (PSCs) are the main source of pancreatic fibrosis. Octreotide
is widely used as a prophylactic for postoperative complications in pancreas transplant
recipients. Recent studies have shown that it can inhibit liver fibrosis. This study
investigated the effect of octreotide in pancreas graft fibrosis in rats.
Materials and methods
Isolated PSCs from Sprague Dawley rats were co-cultured with different doses of octreotide
(1.25, 2.5, 5, 10, 20, and 40 ng/mL). PSC proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide at 48, 72, and 96 h. The α-smooth muscle actin (α-SMA) and collagen I expressions
of PSCs were detected by immunohistochemistry and reverse-transcriptase polymerase
chain reaction. Rat heterotopic pancreaticoduodenal transplantation was performed
with and without octreotide treatment (0.01 mg/kg). Pancreas grafts were harvested
at postoperative d 1, 3, 5, and 7. Hematoxylin-eosin staining, Masson’s trichrome
staining, and immunohistochemical staining for α-SMA, collagen I, and tumor growth
factor-β1 (TGF-β1) were performed.
Results
Octreotide at a concentration of >20 ng/mL significantly inhibited PSC activation
and proliferation in vitro. Inflammatory infiltration was reduced in the octreotide group in vivo, and the expression levels of α-SMA, collagen I, and TGF-β1 were also lower, with
statistic significant difference or not. Masson’s trichrome staining showed a decrease
in collagen deposition with octreotide treatment.
Conclusions
Octreotide effectively inhibits PSC activation and proliferation in vitro, but has a limited inhibitory effect on the development of pancreas graft fibrosis.
Key Words
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Article info
Publication history
Published online: July 07, 2011
Accepted:
June 3,
2011
Received:
February 24,
2011
Identification
Copyright
© 2012 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
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- ErratumJournal of Surgical ResearchVol. 180Issue 1
- PreviewIn the November 2011 issue of the Journal of Surgical Research, we regret that in the article, “Sp1 regulates the transcription of BMPR1A” by Fadi S. Dahdaleh , Jennifer C. Carr, Daniel Calva, James R. Howe and James R. Howe (J Surg Res 2011;171:e15-20), an error was made and one of the authors names was left off the list. Both James R. Howe and his son James R. Howe contributed to this work and both should have been listed as authors.
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- ErratumJournal of Surgical ResearchVol. 180Issue 2
- PreviewIn the July 2012 issue of the Journal of Surgical Research, we regret that in the article, “Effects of octreotide on activated pancreatic stellate cell-induced pancreas graft fibrosis in rats,” by Long D, Lu J, Luo L, Guo Y, Li C, Wu W, Shan J, Li L, Li S, Li Y, Lin T, Feng L (J Surg Res 2012;176:248-59), an error was made in the author affiliations. The institution of author Feng L. should be “Key Laboratory of Transplant Engineering and Immunology of Health Ministry of China, Regenerative Medicine Research Center, West China Hospital, Sichuan University”.
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