Transplantation/Immunology| Volume 176, ISSUE 1, P248-259, July 2012

Effects of Octreotide on Activated Pancreatic Stellate Cell-Induced Pancreas Graft Fibrosis in Rats


      Of solid organ transplantations, pancreas transplantation is associated with the highest incidence of pancreatic fibrosis in the early post-transplantation period. Activated pancreatic stellate cells (PSCs) are the main source of pancreatic fibrosis. Octreotide is widely used as a prophylactic for postoperative complications in pancreas transplant recipients. Recent studies have shown that it can inhibit liver fibrosis. This study investigated the effect of octreotide in pancreas graft fibrosis in rats.

      Materials and methods

      Isolated PSCs from Sprague Dawley rats were co-cultured with different doses of octreotide (1.25, 2.5, 5, 10, 20, and 40 ng/mL). PSC proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide at 48, 72, and 96 h. The α-smooth muscle actin (α-SMA) and collagen I expressions of PSCs were detected by immunohistochemistry and reverse-transcriptase polymerase chain reaction. Rat heterotopic pancreaticoduodenal transplantation was performed with and without octreotide treatment (0.01 mg/kg). Pancreas grafts were harvested at postoperative d 1, 3, 5, and 7. Hematoxylin-eosin staining, Masson’s trichrome staining, and immunohistochemical staining for α-SMA, collagen I, and tumor growth factor-β1 (TGF-β1) were performed.


      Octreotide at a concentration of >20 ng/mL significantly inhibited PSC activation and proliferation in vitro. Inflammatory infiltration was reduced in the octreotide group in vivo, and the expression levels of α-SMA, collagen I, and TGF-β1 were also lower, with statistic significant difference or not. Masson’s trichrome staining showed a decrease in collagen deposition with octreotide treatment.


      Octreotide effectively inhibits PSC activation and proliferation in vitro, but has a limited inhibitory effect on the development of pancreas graft fibrosis.

      Key Words

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          In the November 2011 issue of the Journal of Surgical Research, we regret that in the article, “Sp1 regulates the transcription of BMPR1A” by Fadi S. Dahdaleh , Jennifer C. Carr, Daniel Calva, James R. Howe and James R. Howe (J Surg Res 2011;171:e15-20), an error was made and one of the authors names was left off the list. Both James R. Howe and his son James R. Howe contributed to this work and both should have been listed as authors.
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      • Erratum
        Journal of Surgical ResearchVol. 180Issue 2
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          In the July 2012 issue of the Journal of Surgical Research, we regret that in the article, “Effects of octreotide on activated pancreatic stellate cell-induced pancreas graft fibrosis in rats,” by Long D, Lu J, Luo L, Guo Y, Li C, Wu W, Shan J, Li L, Li S, Li Y, Lin T, Feng L (J Surg Res 2012;176:248-59), an error was made in the author affiliations. The institution of author Feng L. should be “Key Laboratory of Transplant Engineering and Immunology of Health Ministry of China, Regenerative Medicine Research Center, West China Hospital, Sichuan University”.
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