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Psychological stress may contribute to temporomandibular joint disorder in rats

  • Author Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Gaoyi Wu
    Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Affiliations
    Department of Stomatology, Jinan General Military Hospital, Jinan, China
    Search for articles by this author
  • Author Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Lei Chen
    Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Affiliations
    Department of Orthodontics, Jinan Stomatological Hospital, Jinan, China
    Search for articles by this author
  • Author Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Huang Fei
    Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
    Affiliations
    Department of General Dentistry and Emergency, School of Stomatology, Fourth Military Medical University, Xi'an, China
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  • Yucheng Su
    Affiliations
    Department of Stomatology, Peking Union Medical College Hospital, Beijing, China
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  • Guoxiong Zhu
    Correspondence
    Corresponding author. Department of Stomatology, Jinan General Military Hospital, Jinan, China. Tel./fax: +0086 053151665829.
    Affiliations
    Department of Stomatology, Jinan General Military Hospital, Jinan, China
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  • Yongjin Chen
    Correspondence
    Corresponding author. Department of General Dentistry and Emergency, School of Stomatology, Fourth Military Medical University, Xi'an, China. Tel./fax: +0086 02984776488.
    Affiliations
    Department of General Dentistry and Emergency, School of Stomatology, Fourth Military Medical University, Xi'an, China
    Search for articles by this author
  • Author Footnotes
    1 Gaoyi Wu, Lei Chen, and Huang Fei contributed equally to this paper and should be regarded as co–first authors.
Published:January 14, 2013DOI:https://doi.org/10.1016/j.jss.2012.12.014

      Abstract

      Background

      Psychological stress is considered a possible pathogenic factor for temporomandibular joint disorders (TMJD), but few reports have supplied direct evidence. This study was designed to observe the effects of psychological stress on the masticatory muscles and condylar processes in rats to directly investigate the role of psychological stress in TMJD morbidity.

      Materials and methods

      A well-established rat communication box model was used to compare the myoelectric profiles of temporal and masseter muscles and condylar microstructure among rats in a control group, a psychological stress group (PS group), and a diazepam (anxiolytic agent) injection group (PS + DI group). Reverse-transcription polymerase chain reaction was also used to analyze the substance P mRNA and calcitonin gene–related peptide mRNA levels expressed in condylar cartilages during different phases of psychological stress.

      Results

      At 1, 3, and 5 wk, both temporal and masseter muscles in the PS group exhibited a significantly higher electrical potential in relaxation than those in the control group (P < 0.01). The electrical potential during contraction of the temporal and masseter muscles was higher than in the relaxation or control group at 1, 3, and 5 wk (P < 0.01). Scanning electron microscopy demonstrated pathologic changes in condylar processes in the PS group that were not observed in the PS + DI group. Reverse-transcription polymerase chain reaction also showed that the expression of substance P and calcitonin gene–related peptide in rat temporomandibular joint was upregulated during each phase of the psychological stress (P < 0.05).

      Conclusions

      Psychological stress may play an important role in the formation of TMJD.

      Keywords

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