Abstract
Background
Mouse models of liver transplantation are powerful tools for biomedical research.
The cuff method is currently the most popular approach for revascularization of mouse
liver grafts, as it is relatively easy to perform hence reducing the anhepatic time.
However, the use of cuffs may induce a tissue reaction, causing chronic obstruction
of anastomosed vessels, leading to portal hypertension. Here, we applied the suture
technique for arterialized liver transplantation in mice.
Materials and methods
Liver transplantation was performed on 14 pairs of C57BL/6 mice. All hepatic vessels
were anastomosed by sewing. The bile duct was connected with a stent. The liver grafts
were harvested for histology on day 30 after surgery. Serum aspartate transaminase,
alkaline phosphatase and bilirubin were measured at d 3, 7, and 30 after implantation.
Results
With a mean anhepatic time of 25.78 ± 3 min, the survival rate was 86% (n = 14) at 30 d following surgery. During this period, no significant liver injury
was observed as assessed by serum markers and histology. Survival remained stable
when grafts were exposed to 6 h cold ischemia prior to implantation. Vessel examination
at the end of the studied period revealed an intact patency and a lack of collateral
vessel growth.
Conclusion
Arterialized liver transplantation with sewed revascularization in mice is technically
feasible. Both sewing and arterialization seem to be important factors promoting the
survival of mouse recipients. The mouse model of suture arterialized orthotopic liver
transplantation provides a novel tool for modern transplantation research and might
be particularly suited for studies requiring longer-term survival of recipients.
Keywords
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Article info
Publication history
Published online: April 01, 2013
Accepted:
March 7,
2013
Received in revised form:
February 8,
2013
Received:
June 18,
2012
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
