Abstract
Background
Significant consequences of severe burn include skeletal muscle atrophy and heterotopic
ossification (HO). The cellular mechanisms underlying either of these conditions are
not known. Whether the functionality of satellite cells stem cells resident in skeletal
muscle is affected by changes in circulatory factors following burn was determined
to better understand their role in atrophy and HO.
Materials and methods
Serum (20%) from sham-treated animals or burned animals (40% total body surface area
full-thickness burn) was used to culture satellite cells isolated from either sham
or burn animals. Satellite cells were separated based on fiber type (i.e., fast-twitch
or slow-twitch in some cases). To gain greater insight into the potential role for
satellite cells in controlling muscle mass following burn, the effect of serum taken
from burn animals on satellite cell proliferation, migration, and myogenic differentiation
was evaluated. Osteogenic differentiation was assessed to evaluate the potential of
satellite cells to contribute to HO.
Results
Burn serum (BS) increased the proliferative capacity of cells from fast-twitch muscle,
and the migratory capacity of satellite cells taken from both fast- and slow-twitch
muscles. BS increased both the myogenic and osteogenic differentiation of satellite
cells taken from both sham and burn animals.
Conclusions
The unexpected increase in myogenic functionality of satellite cells with BS is difficult
to rectify, given the degree of atrophy that occurs. However, the increased osteogenic
capacity of satellite cells with BS suggests they may play a role in burn-induced
HO.
Keywords
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Article info
Publication history
Published online: April 08, 2013
Accepted:
March 14,
2013
Received in revised form:
March 5,
2013
Received:
January 13,
2013
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
