Abstract
Background
Lung ischemia-reperfusion (I/R) injury plays an important role in lung transplantation.
Less well known is the role of sildenafil in lung I/R injury; therefore, we attempted
to determine whether sildenafil could alleviate lung apoptosis and tissue injury in
a rat model.
Methods
Forty male Sprague-Dawley rats were randomized into four groups: saline + sham, saline
+ I/R, sildenafil + sham, and sildenafil + I/R groups. Three hours before the operation, each
rat received normal saline or sildenafil (10 mg/kg) by lavage. The animals designed
to I/R injury were subjected to 2 h of ischemia induced by occlusion of left pulmonary
artery, veins, and bronchus, followed by reperfusion for 2 h. The lung tissue was
harvested for the analysis of the expression of Bax, Bcl-2, p53, caspase 3, tumor
necrosis factor (TNF)-alpha, interleukin (IL)-6, and wet/dry (W/D) weight ratio.
Results
Compared with the saline + sham group, the saline + I/R group had significant increases
in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α, and W/D weight ratio but a decrease
in Bcl-2 (P < 0.05). Compared with the saline + I/R group, sildenafil + I/R group had significant
decreases in Bax, p53, Bax/Bcl-2 ratio, caspase 3, IL-6, TNF-α level, and W/D weight
ratio but an increase in Bcl-2 expression (P < 0.05). Compared with the sildenafil + sham group, there were significant increases
in p53 and TNF-α expression in the sildenafil + I/R group (P < 0.05).
Conclusions
Pretreatment with sildenafil alleviates lung apoptosis and tissue injury in a rat
model.
Keywords
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Article info
Publication history
Published online: August 02, 2013
Accepted:
July 5,
2013
Received in revised form:
May 30,
2013
Received:
February 2,
2013
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
