Abstract
Background
Activated mast cells are involved in the pathogenesis of intestinal ischemia–reperfusion
(I/R)-related injury. Dexamethasone has been widely used to protect organs from I/R
injury. This study was conducted to investigate the impact of treatment with dexamethasone
at different stages of the II/R process on mast cell infiltration and activity and
intestinal injury.
Methods
Kunming mice were randomized and subjected to a sham surgery or the II/R induction
by clamping the superior mesenteric artery for 30 min and then reperfusion. During
the II/R induction, the mice were treated intravenously with dexamethasone (10 mg/kg)
for 30 min before ischemia (pretreatment group), at 5 min after clamping the superior
mesenteric artery (isc-treatment group), or at the beginning of perfusion (rep-treatment
group), respectively. The levels of intestinal injury, mast cell infiltration and
activity, tumor necrosis factor α (TNFα) and myeloperoxidase (MPO) activity in the
intestines, and mouse survival rates were measured.
Results
The death rates, levels of intestinal injury, mast cell infiltration and activity,
and tumor necrosis factor α and myeloperoxidase activity in the intestinal tissues
from the II/R group were similar to those from the isc-treatment and rep-treatment
groups of mice and were significantly higher than those from the sham group. In contrast,
pretreatment with dexamethasone significantly mitigated the II/R-induced mast cell
infiltration and activity, inflammation, and intestinal injury and reduced the death
rates in mice.
Conclusions
Pretreatment with dexamethasone inhibits II/R injury by reducing mast cell–related
inflammation in mice.
Keywords
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Article info
Publication history
Published online: August 16, 2013
Accepted:
July 25,
2013
Received in revised form:
July 24,
2013
Received:
May 24,
2013
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
