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Dexamethasone pretreatment alleviates intestinal ischemia–reperfusion injury

  • Author Footnotes
    1 These authors contributed equally to this study.
    Wenhua Zhang
    Footnotes
    1 These authors contributed equally to this study.
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

    Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou, China
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this study.
    Jibin Xing
    Footnotes
    1 These authors contributed equally to this study.
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
    Search for articles by this author
  • Dezhao Liu
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
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  • Xiaoliang Gan
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
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  • Wanling Gao
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
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  • Ziqing Hei
    Correspondence
    Corresponding author. Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou 510630, China. Tel.: +86 20 87580867; fax: +86 20 85253336.
    Affiliations
    Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this study.
Published:August 16, 2013DOI:https://doi.org/10.1016/j.jss.2013.07.049

      Abstract

      Background

      Activated mast cells are involved in the pathogenesis of intestinal ischemia–reperfusion (I/R)-related injury. Dexamethasone has been widely used to protect organs from I/R injury. This study was conducted to investigate the impact of treatment with dexamethasone at different stages of the II/R process on mast cell infiltration and activity and intestinal injury.

      Methods

      Kunming mice were randomized and subjected to a sham surgery or the II/R induction by clamping the superior mesenteric artery for 30 min and then reperfusion. During the II/R induction, the mice were treated intravenously with dexamethasone (10 mg/kg) for 30 min before ischemia (pretreatment group), at 5 min after clamping the superior mesenteric artery (isc-treatment group), or at the beginning of perfusion (rep-treatment group), respectively. The levels of intestinal injury, mast cell infiltration and activity, tumor necrosis factor α (TNFα) and myeloperoxidase (MPO) activity in the intestines, and mouse survival rates were measured.

      Results

      The death rates, levels of intestinal injury, mast cell infiltration and activity, and tumor necrosis factor α and myeloperoxidase activity in the intestinal tissues from the II/R group were similar to those from the isc-treatment and rep-treatment groups of mice and were significantly higher than those from the sham group. In contrast, pretreatment with dexamethasone significantly mitigated the II/R-induced mast cell infiltration and activity, inflammation, and intestinal injury and reduced the death rates in mice.

      Conclusions

      Pretreatment with dexamethasone inhibits II/R injury by reducing mast cell–related inflammation in mice.

      Keywords

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