Abstract
Background
A number of therapies are being developed that use microvessels isolated from adipose
tissue (microvascular fragments [MVFs]) to improve tissue perfusion and implant survival.
Because it has been demonstrated that stem cells are associated with microvessels,
the purpose of these studies was to gain further insight into the stem cells associated
with MVFs to better understand their therapeutic potential.
Materials and methods
Cells derived from MVF explants were compared with adipose-derived stem cells (ASCs)
based on the expression of cell surface proteins for mesenchymal stem cells and their
capacity for angiogenic, neurogenic, adipogenic, and osteogenic differentiation.
Results
The expression of cell surface proteins for mesenchymal stem cell markers was similar
between MVF-derived cells and ASCs; however, the increase in markers consistent with
endothelial cells and pericytes was accompanied by an improved ability to form capillary-like
networks when cultured on matrigel. MVF-derived cells had increased neuregulin, leptin,
and osteopontin expression compared with ASCs when exposed to neurogenic, adipogenic,
and osteogenic induction media, respectively.
Conclusions
The stem cell functionality of cells derived from MVFs is retained after their isolation.
This helps to explain the ability of MVFs to improve tissue perfusion and has implications
for the use of MVFs as a means to deliver stem cells within their niche.
Keywords
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Article info
Publication history
Published online: May 24, 2014
Accepted:
May 16,
2014
Received in revised form:
May 13,
2014
Received:
February 4,
2014
Identification
Copyright
Published by Elsevier Inc.
