Hydrogen water alleviates lung injury induced by one-lung ventilation



      With the development of thoracic surgeries, one-lung ventilation (OLV) has been routinely used to facilitate surgical exposure. However, OLV can cause lung injury during the surgical process and becomes an important factor affecting the outcomes. To date, effective treatments for the prevention of lung injury caused by OLV are lacking. Hydrogen has been demonstrated to have effective protection against tissue injuries caused by oxidative stress, inflammation, and apoptosis. This study investigated the efficacy of hydrogen water consumption on the prevention of lung injury induced by OLV in rats.

      Materials and Methods

      Male Sprague–Dawley rats (n = 32, 240–260 g) were divided randomly into the following four groups: sham group, sham + H2 group, OLV group, OLV + H2 group. The rats drank hydrogen water or degassed hydrogen water for 4 wk before the operation and received OLV for 60 min and two-lung ventilation for 60 min. Lung tissues were assayed for wet-to-dry ratio, oxidative stress variables, proinflammatory cytokines, and hematoxylin–eosin staining.


      Hydrogen water consumption reduced wet-to-dry weight ratio, malondialdehyde and myeloperoxidase activity and decreased the concentration of TNF-α, IL-1β, and IL-6 in the lung tissues compared with sham group and sham + H2 group. Hydrogen water consumption further attenuated NF-κB activation and caused histopathologic alterations.


      Our data demonstrated that hydrogen water consumption ameliorated OLV-induced lung injury, and it may exert its protective role by its anti-inflammation, antioxidation and reducing NF-κB activity in the lung tissues.


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        • Amato M.B.
        • Barbas C.S.
        • Medeiros D.M.
        • et al.
        Effect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome.
        N Engl J Med. 1998; 338: 347
        • Jeon K.
        • Yoon J.W.
        • Suh G.Y.
        • et al.
        Risk factors for post-pneumonectomy acute lung injury/acute respiratory distress syndrome in primary lung cancer patients.
        Anaesth Intensive Care. 2009; 37: 14
        • Baudouin S.V.
        Lung injury after thoracotomy.
        Br J Anaesth. 2003; 91: 132
        • Sun Q.
        • Cai J.
        • Liu S.
        • et al.
        Hydrogen-rich saline provides protection against hyperoxic lung injury.
        J Surg Res. 2011; 165: e43
        • Zhang J.-Y.
        • Liu C.
        • Zhou L.
        • et al.
        A review of hydrogen as a new medical therapy.
        Hepatogastroenterology. 2012; 59: 1026
        • Ohsawa I.
        • Nishimaki K.
        • Yamagata K.
        • Ishikawa M.
        • Ohta S.
        Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice.
        Biochem Biophys Res Commun. 2008; 377: 1195
        • Buchholz B.M.
        • Kaczorowski D.J.
        • Sugimoto R.
        • et al.
        Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury.
        Am J Transplant. 2008; 8: 2015
        • Huang C.S.
        • Kawamura T.
        • Lee S.
        • et al.
        Hydrogen inhalation ameliorates ventilator-induced lung injury.
        Crit Care. 2010; 14: R234
        • Li H.
        • Zhou R.
        • Liu J.
        • et al.
        Hydrogen-rich saline attenuates lung ischemia-reperfusion injury in rabbits.
        J Surg Res. 2012; 174: e11
        • Liu C.
        • Wu Q.
        • Li Q.
        • et al.
        Mesenteric lymphatic ducts ligation decreases the degree of gut-induced lung injury in a portal vein occlusion and reperfusion canine model.
        J Surg Res. 2009; 154: 45
        • Kostopanagiotou G.
        • Avgerinos E.
        • Costopanagiotou C.
        • et al.
        Acute lung injury in a rat model of intestinal ischemia-reperfusion: the potential time depended role of phospholipases A(2).
        J Surg Res. 2008; 147: 108
        • Cheng Y.D.
        • Gao Y.
        • Zhang H.
        • Duan C.J.
        • Zhang C.F.
        Effects of OLV preconditioning and postconditioning on lung injury in thoracotomy.
        Asian J Surg. 2014; 37: 80
        • Eguchi T.
        • Yoshida K.
        • Kondo R.
        • et al.
        Sivelestat prevents cytoskeletal rearrangements in neutrophils resulting from lung re-expansion following one-lung ventilation during thoracic surgery.
        Inflammation. 2013; 36: 1479
        • Bastin A.J.
        • Sato H.
        • Davidson S.J.
        • Quinlan G.J.
        • Griffiths M.J.
        Biomarkers of lung injury after one-lung ventilation for lung resection.
        Respirology. 2011; 16: 138
        • Huang C.S.
        • Kawamura T.
        • Peng X.
        • et al.
        Hydrogen inhalation reduced epithelial apoptosis in ventilator-induced lung injury via a mechanism involving nuclear factor-kappa B activation.
        Biochem Biophys Res Commun. 2011; 408: 253
        • Wang C.
        • Li J.
        • Liu Q.
        • et al.
        Hydrogen-rich saline reduces oxidative stress and inflammation by inhibit of JNK and NF-kappaB activation in a rat model of amyloid-beta-induced Alzheimer's disease.
        Neurosci Lett. 2011; 491: 127
        • Olivant Fisher A.
        • Husain K.
        • Wolfson M.R.
        • et al.
        Hyperoxia during one lung ventilation: inflammatory and oxidative responses.
        Pediatr Pulmonol. 2012; 47: 979
        • Leite C.F.
        • Calixto M.C.
        • Toro I.F.
        • Antunes E.
        • Mussi R.K.
        Characterization of pulmonary and systemic inflammatory responses produced by lung re-expansion after one-lung ventilation.
        J Cardiothorac Vasc Anesth. 2012; 26: 427
        • Gothard J.
        Lung injury after thoracic surgery and one-lung ventilation.
        Curr Opin Anaesthesiol. 2006; 19: 5
        • Davidovich N.
        • DiPaolo B.C.
        • Lawrence G.G.
        • Chhour P.
        • Yehya N.
        • Margulies S.S.
        Cyclic stretch-induced oxidative stress increases pulmonary alveolar epithelial permeability.
        Am J Respir Cell Mol Biol. 2013; 49: 156
        • Ohsawa I.
        • Ishikawa M.
        • Takahashi K.
        • et al.
        Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals.
        Nat Med. 2007; 13: 688
        • Sato Y.
        • Kajiyama S.
        • Amano A.
        • et al.
        Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice.
        Biochem Biophys Res Commun. 2008; 375: 346
        • Goodman R.B.
        • Pugin J.
        • Lee J.S.
        • Matthay M.A.
        Cytokine-mediated inflammation in acute lung injury.
        Cytokine Growth Factor Rev. 2003; 14: 523
        • Qiu X.
        • Li H.
        • Tang H.
        • et al.
        Hydrogen inhalation ameliorates lipopolysaccharide-induced acute lung injury in mice.
        Int Immunopharmacol. 2011; 11: 2130
        • Slutsky A.S.
        • Ranieri V.M.
        Ventilator-induced lung injury.
        N Engl J Med. 2013; 369: 2126
        • Lai C.C.
        • Liu W.L.
        • Chen C.M.
        Glutamine attenuates acute lung injury caused by acid aspiration.
        Nutrients. 2014; 6: 3101
        • Zhang J.
        • Wu Q.
        • Song S.
        • et al.
        Effect of hydrogen-rich water on acute peritonitis of rat models.
        Int Immunopharmacol. 2014; 21: 94
        • Chen H.
        • Sun Y.P.
        • Li Y.
        • et al.
        Hydrogen-rich saline ameliorates the severity of l-arginine-induced acute pancreatitis in rats.
        Biochem Biophys Res Commun. 2010; 393: 308
        • Liu C.
        • Kurokawa R.
        • Fujino M.
        • Hirano S.
        • Sato B.
        • Li X.K.
        Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes.
        Sci Rep. 2014; 4: 5485