Abstract
Background
Aortic aneurysms in the viscerorenal-segment are nowadays treatable by endovascular
means. Previously, new endograft techniques were only tested in healthy animals. We
aimed to establish a new large animal model for testing complex endovascular stent
techniques preclinically.
Methods
In sheep, four juxtarenal and two type IV thoracoabdominal aortic aneurysms were surgically
created via a retroperitoneal approach. Two pieces out of a 10 × 15-cm bovine pericardial
patch were sewn with the healthy aorta longitudinally. The viscerorenal segment was
clamped, and the aorta was incised longitudinally. Then, the patches were longitudinally
sewn together. In the meantime, antegrade flow through the native part of the aorta
was already established by tangential clamping. Computed tomography angiography was
performed after 4, 8, and 52 wk.
Results
Technical success was 100%. The median surgical procedure time was 3 h, the median
blood loss was 210 mL, and the viscerorenal-segment clamping time was 2-4 min. The
animals started drinking 1 h after arousal from anesthesia. One animal died after
1 wk because of delayed bleeding and another died after 1 y because of aneurysm rupture
by a secondary bacterial infection. Four animals survived. The proximal landing zone
diameter and the clock position of the vessel were stable over 52 wk.
Conclusions
Surgical creation of an aortic aneurysm in the viscerorenal-segment in sheep was successful,
without an ischemia/reperfusion injury. This animal model offers a new platform for
evaluating innovative endovascular therapy options in vivo.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of Surgical ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- A fenestrated covered suprarenal aortic stent.Eur J Vasc Endovasc Surg. 1999; 18: 445-449
- Endovascular management of juxtarenal aneurysms with fenestrated endovascular grafting.J Vasc Surg. 2004; 39: 279-287
- Treatment of short-necked infrarenal aortic aneurysms with fenestrated stent-grafts: short-term results.Eur J Vasc Endovasc Surg. 2004; 27: 477-483
- Editor's choice – management of descending thoracic aorta diseases.Eur J Vasc Endovasc Surg. 2017; 53: 4-52
- A canine model of proximal descending thoracic aortic aneurysm created with an autologous pericardial patch.Ann Thorac Cardiovasc Surg. 2013; 19: 131-135
- Antegrade in situ fenestration of aortic stent graft: in-vivo experiments using a pig model.Acta Radiol. 2012; 53: 754-758
- Imalytics preclinical: interactive analysis of biomedical volume data.Theranostics. 2016; 6: 328-341
- Hybrid microCT-FMT imaging and image analysis.J Vis Exp. 2015; 100: e52770
- A novel swine model of abdominal aortic aneurysm.J Vasc Surg. 2018; (https://doi.org/10.1016/j.jvs.2018.09.057. [Epub ahead of print])
- A novel chronic advanced stage abdominal aortic aneurysm murine model.J Vasc Surg. 2017; 66: 232-242.e234
- Patch angioplasty in the rat aorta or inferior vena cava.J Vis Exp. 2017; 120 (https://doi.org/10.3791/55253. [Epub ahead of print])
- In vivo evaluation of customized aortic repair using a novel survival model.Eur J Vasc Endovasc Surg. 2016; 52: 166-172
- Open construction of experimental abdominal aortic aneurysm swine models with Dacron patch for evaluating endovascular aneurysm repair techniques.Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2014; 36: 92-97
- Endovascular aortic aneurysm repair with stent-grafts: experimental models can reproduce endoleaks.J Vasc Interv Radiol. 2004; 15: 971-979
- A new animal model for abdominal aortic aneurysms:initial results using a multiple-wire stent.Eur J Vasc Endovasc Surg. 1996; 11: 90-97
- A new model of abdominal aortic aneurysm with gastric serosa patch: surgical technique and short-term evaluation.J Invest Surg. 2006; 19: 97-104
- Postoperative ileus is maintained by intestinal immune infiltrates that activate inhibitory neural pathways in mice.Gastroenterology. 2003; 125: 1137-1147
- Gastrointestinal mucosal injury in experimental models of shock, trauma, and sepsis.Crit Care Med. 1991; 19: 627-641
- The impact of selective visceral perfusion on intestinal macrohemodynamics and microhemodynamics in a porcine model of thoracic aortic cross-clamping.J Vasc Surg. 2012; 56: 149-158
- The role of the gastrointestinal tract in postinjury multiple organ failure.Am J Surg. 1999; 178: 449-453
- Anatomic changes of target vessels after fenestrated and branched aortic aneurysm repair.J Cardiovasc Surg (Torino). 2014; 55: 115-121
Article info
Publication history
Published online: March 29, 2019
Accepted:
February 25,
2019
Received in revised form:
January 23,
2019
Received:
November 8,
2018
Identification
Copyright
© 2019 Elsevier Inc. All rights reserved.
