Highlights:
- •SNHG14 was upregulated, and miR-124-3p was downregulated by LPS in human lung epithelial cells in vitro.
- •Silencing of SNHG14 and overexpression of miR-124-3p could attenuate LPS-induced apoptosis and inflammation in A549 cells.
- •miR-124-3p, sponged by SNHG14, negatively regulated TGFBR2 expression via target binding.
- •There was a novel SNHG14/miR-124-3p/TGFBR2 circuit in ALI.
Abstract
Background
Emerging evidence show that long noncoding RNAs (lncRNAs) are crucial regulators in
pathophysiology of acute lung injury (ALI). Small nucleolar RNA host gene 14 (SNHG14)
is a novel oncogenic lncRNA, and has been associated with inflammation-related cell
injuries. Thus, we wondered the role and mechanism of SNHG14 in lipopolysaccharides
(LPS)-induced ALI cell model.
Methods
Expression of SNHG14, miRNA (miR)-124-3p, and transforming growth factor β type 2
receptor (TGFBR2) was detected by RT-qPCR and western blotting. Cell apoptosis was
determined by methyl thiazolyl tetrazolium assay, flow cytometry, western blotting,
and lactate dehydrogenase activity kit. Inflammation was measured by enzyme-linked
immunosorbent assay. The interaction among SNHG14, miR-124-3p, and TGFBR2 was validated
by dual-luciferase reporter assay and RNA immunoprecipitation.
Results
LPS administration attenuated human lung epithelial cell viability and B-cell lymphoma-2
expression, but augmented apoptosis rate, cleaved-caspase-3 expression, lactate dehydrogenase
activity, and secretions of tumor necrosis factor-α, interleukin-1β, and IL-6 in A549 cells.
Thus, LPS induced A549 cells apoptosis and inflammation, wherein SNHG14 was upregulated
and miR-124-3p was downregulated. However, silencing SNHG14 could suppress LPS-induced
apoptosis and inflammation depending on upregulating miR-124-3p via target binding.
Similarly, overexpressing miR-124-3p attenuated LPS-induced A549 cells injury through
inhibiting its downstream target TGFBR2. Furthermore, SNHG14 knockdown could also
affect TGFBR2 expression via miR-124-3p.
Conclusions
SNHG14 knockdown prevents A549 cells from LPS-induced apoptosis and inflammation through
regulating miR-124-3p and TGFBR2, suggesting a novel SNHG14/miR-124-3p/TGFBR2 circuit
in alveolar epithelial cells on the set of ALI.
Keywords
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Article info
Publication history
Published online: February 27, 2021
Accepted:
October 31,
2020
Received in revised form:
September 15,
2020
Received:
May 14,
2020
Footnotes
Funding: No funding was received.
Availability of data and materials: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate: The present study was approved by the ethical review committee of Linyi Central Hospital. Written informed consent was obtained from all enrolled patients.
Patient consent for publication: Not applicable.
Identification
Copyright
© 2020 Elsevier Inc. All rights reserved.
