Abstract
Background
Acute mesenteric ischemia arises through sudden interruption of mesenteric blood flow,
mostly due to an occlusion of the superior mesenteric artery and is associated with
a high mortality of approximately 50% to 90%. In previous studies, the single application
of β-alanine or aprotinin caused an ameliorated intestinal damage but without any
systemic effects.
Methods
To analyze the combined effect of β-alanine and aprotinin on acute ischemia and reperfusion
of the small intestine, a model with anesthetized rats was used. Ischemia and reperfusion
were initiated by occluding and reopening the superior mesenteric artery. After 120 min
of ischemia and 180 min of reperfusion, the intestine was analyzed for tissue damage,
the activity of the saccharase, and accumulation of granulocytes. In addition, systemic
and metabolic as well as inflammatory parameters were measured in blood at certain
points in time.
Results
The combination of β-alanine and aprotinin resulted in a clearly stabilized mean arterial
blood pressure and blood glucose level during the reperfusion period. Furthermore,
the combined administration resulted in significantly reduced tissue damage parameters,
cytokine and cell-free hemoglobin concentrations in blood plasma. In addition, the
damage to the small intestine was significantly attenuated, so that the animals ultimately
survived the entire test period because of the administration of both substances.
Conclusions
Overall, the simultaneous application of both substances leads to a synergistic protection
without the occurrence of undesirable side effects. The combined usage of β-alanine
and aprotinin can be seen as a promising approach to inhibit the onset of acute mesenteric
ischemia.
Keywords
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Article info
Publication history
Published online: February 24, 2021
Accepted:
January 25,
2021
Received in revised form:
January 10,
2021
Received:
July 23,
2020
Identification
Copyright
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