ABSTRACT
Background
Spinal Cord ischemia-reperfusion injury (SCII) is one of the most destructive complications
in thoracic-abdominal aortic surgery, which can cause physical abnormalities, paralysis
and even brain death. Evidence has shown that perillaldehyde (PAH) can ameliorate
rat's cerebra ischemia-reperfusion injury. However, the effect of PAH on SCII remains
unknown.
Methods
The current study established SCII rat models and oxygen and glucose deprivation/reoxygenation-induced
BV2 microglia models to explore whether PAH could alleviate SCII symptoms and to investigate
underlying mechanism.
Results
SCII rats underwent severe neurologic motor dysfunction and histopathologic injury
compared with the normal rats, which are exhibited by loss of motor neurons and decrease
of nissl bodies. Treatment with PAH significantly ameliorated motor dysfunction and
neuron damage. PAH downregulated the expression of NLR family pyrin domain containing
3, cleaved/pro caspase-1, interleukin-1β and interleukin-18 in spinal cord tissues
of SCII rats. Besides, the contents of oxidative stress-related factors superoxide
dismutase, manganese-dependent superoxide dismutase, catalase and glutathione peroxidase
were significantly increased and malondialdehyde content was decreased after PAH treatment.
PAH treatment upregulated the expression of nuclear factor-E2-related factor 2 and
heme oxygenase-1 in spinal cord tissues of SCII rats. Our in vitro study confirmed that PAH inhibited microglial activation by activating the nuclear
factor-E2-related factor 2/heme oxygenase-1 pathway, exhibited by alleviated inflammation
and oxidative stress.
Conclusions
This study elucidates that PAH has the potential value for treating SCII, which provides
an experimental basis for clinical trials in the future.
Keywords
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Article info
Publication history
Published online: August 13, 2021
Accepted:
June 11,
2021
Received in revised form:
May 31,
2021
Received:
December 28,
2020
Identification
Copyright
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