Highlights
- •Presepsin (P-SEP) was first discovered in 2002; however, there have been no reports on serum P-SEP level in mice.
- •This study used a P-SEP enzyme-linked immunosorbent assay kit to evaluate the changes in serum P-SEP level in mouse sepsis models compared with other inflammatory markers.
- •Serum P-SEP levels increased earlier than other inflammatory markers in the mouse cecal ligation and puncture model. In contrast, serum P-SEP levels were not elevated in the CL and LPS-induced sepsis models.
- •Mouse P-SEP is elevated early in infection and more specific to bacterial infection compared with other biomarkers.
Abstract
Introduction
Since its discovery in 2002, presepsin (P-SEP) has been reported to be useful in the
early diagnosis of sepsis and has been evaluated in many clinical studies. However,
as antibodies that bind to mouse P-SEP were previously unavailable, serum P-SEP levels
in mice are limited. This study used a P-SEP enzyme-linked immunosorbent assay kit
to evaluate the changes in serum P-SEP levels in mouse sepsis models compared with
changes in other inflammatory markers and determine whether P-SEP can function as
a biomarker specific to bacterial infections.
Methods
Sepsis was induced in mice via cecal ligation and puncture (CLP), induction with lipopolysaccharide
(LPS), and cecal ligation (CL) model was created as a control for the CLP model, following
which clinical biomarkers (P-SEP, C-reactive protein, and procalcitonin) were evaluated.
Results
The 48-h survival rates in the CLP, CL, and LPS-induced sepsis models were 67%, 89%,
and 57%, respectively. Serum C-reactive protein levels did not increase in the CLP
and CL models within 24 h but significantly increased in the LPS-induced sepsis model.
Serum procalcitonin levels increased in the CLP and CL models and especially increased
in the LPS-induced sepsis model. In contrast, an increase in serum P-SEP level was
found in the CLP model at 6 h compared with those at baseline, the CL, and LPS-induced
sepsis models.
Conclusions
Mouse P-SEP is elevated early in infection and more specific to bacterial infection
compared with other biomarkers.
Keywords
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Article info
Publication history
Published online: November 25, 2022
Accepted:
October 15,
2022
Received in revised form:
September 3,
2022
Received:
February 21,
2022
Identification
Copyright
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