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Specificity of Presepsin as a Biomarker of Bacterial Infection in Mouse Sepsis Models

Published:November 25, 2022DOI:https://doi.org/10.1016/j.jss.2022.10.063

      Highlights

      • Presepsin (P-SEP) was first discovered in 2002; however, there have been no reports on serum P-SEP level in mice.
      • This study used a P-SEP enzyme-linked immunosorbent assay kit to evaluate the changes in serum P-SEP level in mouse sepsis models compared with other inflammatory markers.
      • Serum P-SEP levels increased earlier than other inflammatory markers in the mouse cecal ligation and puncture model. In contrast, serum P-SEP levels were not elevated in the CL and LPS-induced sepsis models.
      • Mouse P-SEP is elevated early in infection and more specific to bacterial infection compared with other biomarkers.

      Abstract

      Introduction

      Since its discovery in 2002, presepsin (P-SEP) has been reported to be useful in the early diagnosis of sepsis and has been evaluated in many clinical studies. However, as antibodies that bind to mouse P-SEP were previously unavailable, serum P-SEP levels in mice are limited. This study used a P-SEP enzyme-linked immunosorbent assay kit to evaluate the changes in serum P-SEP levels in mouse sepsis models compared with changes in other inflammatory markers and determine whether P-SEP can function as a biomarker specific to bacterial infections.

      Methods

      Sepsis was induced in mice via cecal ligation and puncture (CLP), induction with lipopolysaccharide (LPS), and cecal ligation (CL) model was created as a control for the CLP model, following which clinical biomarkers (P-SEP, C-reactive protein, and procalcitonin) were evaluated.

      Results

      The 48-h survival rates in the CLP, CL, and LPS-induced sepsis models were 67%, 89%, and 57%, respectively. Serum C-reactive protein levels did not increase in the CLP and CL models within 24 h but significantly increased in the LPS-induced sepsis model. Serum procalcitonin levels increased in the CLP and CL models and especially increased in the LPS-induced sepsis model. In contrast, an increase in serum P-SEP level was found in the CLP model at 6 h compared with those at baseline, the CL, and LPS-induced sepsis models.

      Conclusions

      Mouse P-SEP is elevated early in infection and more specific to bacterial infection compared with other biomarkers.

      Keywords

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