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Regular Article| Volume 88, ISSUE 2, P78-87, February 2000

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An in Vivo Evaluation of a Chondroitin Sulfate Solution to Prevent Postoperative Intraperitoneal Adhesion Formation

DOI:https://doi.org/10.1006/jsre.1999.5714
An in Vivo Evaluation of a Chondroitin Sulfate Solution to Prevent Postoperative Intraperitoneal Adhesion Formation
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      Abstract

      Purpose. The goal of this study was to determine the efficacy of a single intraperitoneal administration of a chondroitin sulfate solution in preventing postoperative adhesion formation.
      Methods. Twenty-five Sprague–Dawley rats had a 1-cm2 area of cecal serosa abraded. Controls (CON, n = 5) received no treatment, the chondroitin sulfate group (CS, n = 10) received chondroitin sulfate (0.013 g/kg) in 0.9% NaCl intraperitoneally (ip), and vehicle controls (VC, n = 10) received an equal volume of 0.9% NaCl solution ip before the abdomen was closed. All animals were sacrificed on postoperative day 10. The extent of adhesion was quantified according to Mazuji's adhesion grade (0 to 4: 0 = no adhesion and 4 = very dense adhesion) and quantitated after H&E, trichome, and immunohistochemical staining for fibrin and collagen type I and type III using digital image analysis.
      Results. The mean Mazuji's adhesion grade in the CON was 4.0 ± 0.0, in the VC 2.60 ± 0.37, and in the CS 1.3 ± 0.42 (P < 0.01 for CS vs CON and P < 0.05 for CS vs VC comparisons). The mean gray-scale intensity (0–255: 0 = dense amount and 255 = none) of adhesion density in the CON was 105.5 ± 5.5, in the VC 125 ± 15.0, and in the CS 178.3 ± 21.0 (P < 0.01 for CS vs CON and P < 0.05 for CS vs VC comparisons). The mean adjusted intensity stain indices (AISI) for fibrin and collagen type I in the CON were 59 ± 17 and 53 ± 19, in the VC 27 ± 3 and 25 ± 7, and in the CS 16 ± 5 and 6 ± 3, respectively (P < 0.05 between CS and CON comparisons). The AISI of collagen type III was not significant among all the groups (P > 0.1).
      Conclusions. The extent of early postoperative intra-abdominal adhesion formation as determined by gross assessment and from quantitation of fibrin and collagen type I deposition was significantly reduced by a single intraperitoneal administration of a chondroitin sulfate solution.

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      DOI: https://doi.org/10.1006/jsre.1999.5714

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