LPS-stimulated RAW264.7 macrophage CAT-2–mediated l-arginine uptake and nitric oxide biosynthesis is inhibited by omega fatty acid lipid emulsionOmega-3 fatty acid (ω-3 FA) lipid emulsion has been reported to inhibit nitric oxide (NO) production and alter inducible nitric oxide synthase (iNOS) protein expression in lipopolysaccharide (LPS)-stimulated murine macrophages. However, the role of cellular uptake of l-arginine and iNOS transcription in ω-3 FA emulsion-induced inhibition of NO has not been explored. In addition, cationic amino acid transporter-2 (CAT-2) can regulate iNOS activity. The effect of ω-3 FA emulsion on CAT-2 expression is unknown.
Temporary rapid bowel ligation as a damage control adjunct improves survival in a hypothermic traumatic shock swine model with multiple bowel perforationsPrimary intestinal anastomosis is not the right choice for multiple bowel perforations under hemodynamically stable conditions. Our group has employed temporary rapid bowel ligation as a damage control procedure in a hypothermic traumatic shock swine model with multiple bowel perforations and hypothesized that damage control treatment would improve survival in the setting of a damage control surgery.
Exogenous C3 Postpones Complement Exhaustion and Confers Organ Protection in Murine SepsisSepsis in human being is a challenging and life-threatening problem. Complement activation is an essential event in sepsis. The present study observed the dynamic levels of complement components in sepsis and evaluated the role of exogenous complement protein in outcomes. The relationship between complement and inflammatory cytokines was also investigated.
Lymphocyte Depletion and Repopulation in Peripheral Blood and Small Intestine of Cynomolgus Monkeys After Alemtuzumab TreatmentAlemtuzumab has been used as an induction agent in organ transplantation over 10 years, but the effect of alemtuzumab on lymphocytes in small intestine was not clear. We investigate lymphocyte depletion and repopulation phenomena both in peripheral blood and small intestine of cynomolgus monkeys, to assess the model using in preclinical transplantation.